Cell block practices in European cytopathology laboratories

IF 2.6 3区医学 Q3 ONCOLOGY

Cancer Cytopathology Pub Date : 2024-02-07 DOI:10.1002/cncy.22793

Irena Srebotnik Kirbis PhD, Ivana Kholova MD, PhD, Heini Huhtala MSc, Massimo Bongiovanni MD, Margareta Strojan Flezar MD, MIAC, PhD, Chantell Hodgson MSc, Beatrix Cochand-Priollet MD, MIAC, PhD

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Abstract

Background

There are numerous methods and procedures described for the preparation of cell blocks (CBs) from cytological samples. The objective of this study was to determine current practices and issues with CBs in European laboratories.

Methods

A link to an online survey, with 11 questions about CB practices, was distributed to cytology laboratories via participants of United Kingdom National External Quality Assurance Service for Cellular Pathology Techniques and national representatives in the European Federation of Cytology Societies.

Results

A total of 402 laboratories responded completely (337/402, 84%) or partially (65/402, 16%) to the survey by February 4, 2022. The most common CB practice is embedding cell pellets using plasma and thrombin (23.3%), agar (17.1%), Shandon/Epredia Cytoblock (11.4%), HistoGel (7.9%), and Cellient (3.5%). Other methods such as CytoFoam, albumin, gelatin, Cytomatrix, and collodion bags are rarely used (1.0%, 0.7%, 0.7%, 0.3%, and 0.2%, respectively). CBs are also prepared from naturally occurring clots or tissue fragments (29.5%) and cells scraped from unstained or prestained smears (4.4%). The most frequent issues with the CBs in a daily cytology practice are low cellularity (248/402, 62%) and dispersed cells (89/402, 22%), regardless of the CBs preparation method or how the samples for embedding were selected.

Conclusions

There is a great variability in CB practices in European laboratories with low cellular CBs as the main issue. Additional studies are mandatory to evaluate and improve performance and cellular yield of CBs.

Abstract Image

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欧洲细胞病理学实验室的细胞块操作。

背景：从细胞学样本中制备细胞块（CB）的方法和程序有很多。本研究的目的是确定欧洲实验室目前的细胞块制备方法和存在的问题：方法：通过英国国家细胞病理学技术外部质量保证服务机构的参与者和欧洲细胞学协会联合会的国家代表向细胞学实验室分发了一份在线调查的链接，其中包含 11 个有关 CB 操作的问题：截至 2022 年 2 月 4 日，共有 402 家实验室对调查做出了完整回复（337/402，84%）或部分回复（65/402，16%）。最常见的 CB 方法是使用血浆和凝血酶（23.3%）、琼脂（17.1%）、Shandon/Epredia Cytoblock（11.4%）、HistoGel（7.9%）和 Cellient（3.5%）包埋细胞颗粒。其他方法，如 CytoFoam、白蛋白、明胶、Cytomatrix 和胶版袋很少使用（分别为 1.0%、0.7%、0.7%、0.3% 和 0.2%）。制备 CBs 的材料还包括自然形成的血块或组织碎片（29.5%）以及从未染过或预染过的涂片中刮取的细胞（4.4%）。在日常细胞学实践中，无论 CBs 的制备方法或用于包埋的样本是如何选择的，CBs 最常见的问题是细胞度低（248/402，62%）和细胞分散（89/402，22%）：结论：欧洲实验室的 CB 操作存在很大差异，细胞 CB 含量低是主要问题。必须开展更多的研究来评估和提高 CB 的性能和细胞产量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Cytopathology 医学-病理学

CiteScore

7.00

自引率

17.60%

发文量

130

审稿时长

1 months

期刊介绍： Cancer Cytopathology provides a unique forum for interaction and dissemination of original research and educational information relevant to the practice of cytopathology and its related oncologic disciplines. The journal strives to have a positive effect on cancer prevention, early detection, diagnosis, and cure by the publication of high-quality content. The mission of Cancer Cytopathology is to present and inform readers of new applications, technological advances, cutting-edge research, novel applications of molecular techniques, and relevant review articles related to cytopathology.