Evaluation of a single Eporatio® micro-dose in urine and dried blood spots

IF 2.6 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Drug Testing and Analysis Pub Date : 2024-02-06 DOI:10.1002/dta.3651

Carmel E. Heiland, Mikael Lehtihet, Annica Börjesson, Lena Ekström

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Abstract

Recombinant human erythropoietin (rhEPO) has been abused as a performance enhancer in sports for several years, but with advancements in detection methods, even micro-doses can be detected in dried blood spot (DBS) samples. Here, we present the results from an Eporatio® (epoetin theta) micro-dose administration study to detect rhEPO in DBS samples. Five healthy male volunteers received a 15 IU/kg subcutaneous dose of Eporatio®. Urine and DBS samples (Mitra® VAMS and Capitainer® B50) were collected 1, 10, 24, 36, 48 and 72 h after drug administration. After 1 h, all urine samples were negative for rhEPO, whereas 40% of DBS samples were considered suspicious. All samples between 10 and 48 h were suspicious for the presence of Eporatio®, except one urine sample that was negative at 48 h. After 72 h, 40% of urine samples and 60% of DBS samples were suspicious and would have proceeded to a confirmation analysis. DBS is an efficient complementary matrix to urine for detection of rhEPO micro-doses.

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尿液和干血斑中单个 Eporatio® 微量剂量的评估。

重组人促红细胞生成素（rhEPO）作为一种提高运动成绩的药物已被滥用多年，但随着检测方法的进步，即使是微剂量也能在干血斑（DBS）样本中检测出来。在此，我们介绍一项 Eporatio® （epoetin theta）微剂量给药研究的结果，该研究旨在检测干血样中的 rhEPO。五名健康男性志愿者皮下注射了 15 IU/kg 剂量的 Eporatio®。给药后 1、10、24、36、48 和 72 小时收集尿液和 DBS 样本（Mitra® VAMS 和 Capitainer® B50）。1 小时后，所有尿样的 rhEPO 检测结果均为阴性，而 40% 的 DBS 样本被认为可疑。72 小时后，40% 的尿样和 60% 的 DBS 样本为可疑样本，需要进行确认分析。DBS 是尿液检测微量 rhEPO 的有效补充基质。

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来源期刊

Drug Testing and Analysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

5.90

自引率

24.10%

发文量

191

审稿时长

2.3 months

期刊介绍： As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances. In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds). Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.