Serotype Distribution and Multi Locus Sequence Type (MLST) of Erythromycin-Resistant Streptococcus Pneumoniae Isolates in Tehran, Iran.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mohammad Azarsa, Mehrdad Mosadegh, Soheila Habibi Ghahfarokhi, Mohammad Reza Pourmand
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引用次数: 0

Abstract

Background: The number of erythromycin-resistant Streptococcus pneumoniae has significantly increased around the world. The present study aimed to determine the serotype distribution and molecular epidemiology of the erythromycin-resistant Streptococcus pneumoniae (ERSP) isolated from patients with invasive disease.

Methods: A total of 44 Streptococcus pneumoniae isolates were tested for susceptibility to several antimicrobial agents. Additionally, the polymerase chain reaction (PCR) was applied to evaluate ERSP isolates in terms of the presence of erythromycin resistance genes (e.g., ermB and mefA). The isolates were serotyped using the sequential multiplex-PCR method, and molecular epidemiology was assessed through the multilocus sequence typing (MLST) analysis.

Results: The results represented multidrug resistance (MDR) in approximately half of the pneumococcal isolates. Among 22 ERSP isolates, 20 (90.9%) and 12 (56%) ones contained ermB and mefA, respectively. Further, 14 (31.8%), 3 (22.7%), and 19A (18.1%) were the common serotypes among the isolates. No significant correlation was observed between serotypes and erythromycin resistance genes. Furthermore, the MLST results revealed 18 different sequence types (STs), the top ones of which were ST3130 (3 isolates) and ST166 (3 isolates). Population genetic analysis disclosed that CC63 (32%), CC156 (18%), and CC320 (18%) were identified as the predominant clonal complexes.

Conclusions: The ERSP isolates exhibited high genetic diversity. The large frequency of MDR isolates suggests the emergence of high resistant strains, as well as the need to implement vaccination in the immunization schedule of Iran. These accumulating evidences indicate that 13-valent pneumococcal conjugate vaccines provided higher serotype coverage in the ERSP isolates.

伊朗德黑兰耐红霉素肺炎链球菌分离物的血清型分布和多位点序列类型 (MLST)。
背景:耐红霉素肺炎链球菌的数量在全球范围内显著增加。本研究旨在确定从侵袭性疾病患者中分离出的耐红霉素肺炎链球菌(ERSP)的血清型分布和分子流行病学:方法:共检测了 44 株肺炎链球菌分离株对多种抗菌药物的敏感性。此外,还应用聚合酶链反应(PCR)评估了ERSP分离株是否存在红霉素耐药基因(如ermB和mefA)。采用连续多重 PCR 法对分离株进行血清分型,并通过多焦点序列分型(MLST)分析评估分子流行病学:结果:大约一半的肺炎球菌分离株具有耐多药(MDR)性。在 22 个 ERSP 分离物中,分别有 20 个(90.9%)和 12 个(56%)含有 ermB 和 mefA。此外,分离株中常见的血清型有 14 个(31.8%)、3 个(22.7%)和 19A(18.1%)。血清型与红霉素耐药基因之间没有明显的相关性。此外,MLST 结果显示了 18 种不同的序列类型(ST),其中最主要的是 ST3130(3 个分离株)和 ST166(3 个分离株)。群体遗传分析显示,CC63(32%)、CC156(18%)和 CC320(18%)被确定为主要的克隆复合体:结论:ERSP 分离物表现出高度的遗传多样性。MDR 分离物的高频率表明出现了高抗药性菌株,因此需要在伊朗的免疫计划中实施疫苗接种。这些不断积累的证据表明,13 价肺炎球菌结合疫苗对 ERSP 分离物的血清型覆盖率较高。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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