Crocin-loaded Niosomal Nanoparticles Reversing Cytotoxicity and Oxidative Stress in HEK293 Cell Line Exposed to Paraquat: An In vitro Study.

Q2 Pharmacology, Toxicology and Pharmaceutics

Pharmaceutical nanotechnology Pub Date : 2025-01-01 DOI:10.2174/0122117385256493231019045141

Akram Oftadeh Harsin, Sajjad Makhdoomi, Meysam Soleimani, Farzin Firozian, Amir Nili-Ahmadabadi, Akram Ranjbar

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引用次数: 0

Abstract

Background: Paraquat (PQ) is an effective herbicide which is widely used around the world to remove weeds in agriculture. As a water-soluble carotenoid, crocin is a pharmacologically active constituent of C. sativus L. (saffron).

Objectives: In the present study, we investigated the effects of crocin-loaded niosomes (Cro-NIO) compared to free crocin on PQ-induced toxicity in the eukaryotic human embryonic kidney (HEK293) cell line.

Methods: The Cro-NIO was synthesized and characterized. Cell viability was determined using the MTT assay in PQ-exposed HEK293 cell lines. The activities of biochemical markers were quantitatively determined to reveal the potential mechanism of PQ-induced oxidative stress in HEK293 cell line.

Results: The particle size, zeta potential, polydispersity index (PDI), DL, and EE of Cro-NIO were 145.4 ± 19.5 nm, -22.3 ± 3.11 mV, 0.3 ± 0.03, 1.74 ± 0.01%, and 55.3 ± 7.1%, respectively. PQtreated HEK293 cell lines decreased cell viability. The results of oxidative status showed that PQ significantly could increase ROS accumulation, accompanied by a decreasing antioxidant defense system. However, treatment with Cro-NIO, compared to crocin, not only did dose-dependently improve the cell viability but also significantly attenuated the ROS accumulation and increased antioxidant markers.

Conclusion: According to these results, Cro-NIO, compared to crocin, was superior to ameliorating PQ-induced cytotoxicity and oxidative damage in HEK293 cells.

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逆转暴露于百草枯的 HEK293 细胞系的细胞毒性和氧化应激的克罗恩载体纳米颗粒：体外研究。

背景：百草枯（PQ）是一种有效的除草剂，在世界各地被广泛用于农业除草。作为一种水溶性类胡萝卜素，藏红花（C. sativus L.）中的藏红花苷是一种具有药理活性的成分：在本研究中，我们研究了与游离黄花素相比，负载黄花素的niosomes（Cro-NIO）对真核人胚肾（HEK293）细胞系中PQ诱导的毒性的影响：方法：合成了 Cro-NIO 并对其进行了表征。在暴露于 PQ 的 HEK293 细胞系中使用 MTT 法测定细胞活力。定量测定生化标志物的活性，以揭示 PQ 诱导 HEK293 细胞系氧化应激的潜在机制：结果：Cro-NIO的粒径、ZETA电位、多分散指数（PDI）、DL和EE分别为145.4 ± 19.5 nm、-22.3 ± 3.11 mV、0.3 ± 0.03、1.74 ± 0.01%和55.3 ± 7.1%。经 PQ 处理的 HEK293 细胞系的细胞活力下降。氧化状态的研究结果表明，PQ 能显著增加 ROS 的积累，同时降低抗氧化防御系统。然而，与巴豆苷相比，用 Cro-NIO 处理不仅能剂量依赖性地提高细胞活力，还能显著减少 ROS 的积累并增加抗氧化标记物：根据上述结果，与巴豆苷相比，Cro-NIO 在改善 PQ 诱导的 HEK293 细胞细胞毒性和氧化损伤方面更具优势。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmaceutical nanotechnology Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

4.20

自引率

0.00%

发文量

期刊介绍： Pharmaceutical Nanotechnology publishes original manuscripts, full-length/mini reviews, thematic issues, rapid technical notes and commentaries that provide insights into the synthesis, characterisation and pharmaceutical (or diagnostic) application of materials at the nanoscale. The nanoscale is defined as a size range of below 1 µm. Scientific findings related to micro and macro systems with functionality residing within features defined at the nanoscale are also within the scope of the journal. Manuscripts detailing the synthesis, exhaustive characterisation, biological evaluation, clinical testing and/ or toxicological assessment of nanomaterials are of particular interest to the journal’s readership. Articles should be self contained, centred around a well founded hypothesis and should aim to showcase the pharmaceutical/ diagnostic implications of the nanotechnology approach. Manuscripts should aim, wherever possible, to demonstrate the in vivo impact of any nanotechnological intervention. As reducing a material to the nanoscale is capable of fundamentally altering the material’s properties, the journal’s readership is particularly interested in new characterisation techniques and the advanced properties that originate from this size reduction. Both bottom up and top down approaches to the realisation of nanomaterials lie within the scope of the journal.