Simvastatin-Loaded Nanoniosome Protects H9c2 Cells from Oxygen-Glucose Deprivation/ Reperfusion Injury by Downregulating Inflammation.

Q2 Biochemistry, Genetics and Molecular Biology

Iranian Biomedical Journal Pub Date : 2024-01-01 Epub Date: 2023-09-30 DOI:10.61186/ibj.3994

Maryam Naseroleslami, Mahdieh Mehrab Mohseni

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Abstract

Background: Simvastatin (SIM) has anti-inflammatory and antioxidant properties against cardiac ischemia/reperfusion injury (I/RI). However, it suffers from low bioavailability and a short half-life. Nanoniosomes are novel drug delivery systems that may increase SIM effectiveness. The present research evaluates the impact of SIM-loaded nanoniosomes on the oxygen-glucose deprivation/reperfusion (OGD/R) injury model of H9c2 cells.

Methods: Cells were seeded based on five groups: (1) control; (2) OGD/R; (3) OGD/R receiving SIM; (4) OGD/R receiving nanoniosomes; and (5) OGD/R receiving SIM loaded nanoniosomes. OGD/R injury of the H9c2 cells was treated with SIM or SIM loaded nanoniosomes. Cell viability, two inflammatory factors, necroptosis factors, along with HMGB1 and Nrf2 gene expressions were assessed.

Results: The cells treated with SIM loaded nanoniosomes showed a significant elevation in the cell viability and a reduction in HMGB1, Nrf2, TNF-α, IL-1β, RIPK1, and ROCK1 expression levels compared to the OGD/R and SIM groups.

Conclusion: Based on our findings, nanoniosomes could safely serve as a drug delivery system to counterbalance the disadvantages of SIM, resulting in improved aqueous solubility and stability.

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辛伐他汀载体纳米生物体通过抑制炎症保护H9c2细胞免受氧-葡萄糖剥夺/再灌注损伤的影响

背景介绍辛伐他汀对心脏 I/RI 具有抗炎和抗氧化作用。然而，辛伐他汀的生物利用度低，半衰期短。纳米生物体是一种新型给药系统，可提高辛伐他汀的疗效。本研究评估了负载 SIM 的纳米生物体对 H9c2 细胞 OGD/R 损伤模型的影响：将细胞分为五组：(1) 对照组；(2) OGD/R组；(3) 接受SIM的OGD/R组；(4) 接受纳米生物体的OGD/R组；(5) 接受负载SIM的纳米生物体的OGD/R组。H9c2细胞的OGD/R损伤是用SIM或负载SIM的纳米生物体处理的。对细胞活力、两种炎症因子、坏死因子以及 HMGB1 和 Nrf2 基因表达进行了评估：结果：与 OGD/R 组和 SIM 组相比，用负载 SIM 的纳米生物体处理的细胞显示出细胞活力的显著提高，以及 HMGB1、Nrf2、TNF-α、IL-1β、RIPK1 和 ROCK1 表达水平的降低：根据我们的研究结果，纳米生物体可作为一种安全的给药系统来抵消 SIM 的缺点，从而提高水溶性和稳定性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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