Development and optimization of Clitoria teratea synthesized silver nanoparticles and its application to nanogel systems for wound healing.

IF 2.4 4区医学 Q3 CHEMISTRY, MEDICINAL

Drug Development and Industrial Pharmacy Pub Date : 2024-03-01 Epub Date: 2024-02-19 DOI:10.1080/03639045.2024.2308043

Richa Jain, Ruchi Singh, Reena Badhwar, Tinku Gupta, Harvinder Popli

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引用次数: 0

Abstract

Objective: The present research deals with sequential optimization strategy based on Central Composite Design to optimize the process variables for efficient production of Clitoria teratea (CLT) synthesized silver nanoparticles (AgNPs) using biological synthesis.

Methods: Two substantial factors influencing the dependent variables viz UV-visible absorbance, particle size, zeta potential and polydispersity index (PDI) were identified as NaOH concentration, RH concentration, temperature as independent variables. In-vitro and ex-vivo studies of prepared CLT-AgNPs gel and marketed gel were carried out using dialysis membrane and egg membrane, respectively. In addition, antimicrobial study was also performed on the bacterial strains.

Results: The particles size (114 nm), PDI (0.45), and zeta potential (-29.5 mV) of optimized formulation were found, respectively. In-vitro profile of AgNPs from prepared CLT-AgNPs gel was noted (95.6%) in 8 h. It was found that the prepared CLT-AgNPs gel stimulates fibroblast and agranulocytosis development resulting better and timely wound healing.

Conclusions: The prepared CLT-AgNPs gel can be as a potential substitute in the management and treatment of acute and chronic wounds.

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开发和优化 Teratea Clitoria 合成银纳米粒子，并将其应用于伤口愈合纳米凝胶系统。

本研究采用基于中心复合设计的顺序优化策略，以优化工艺变量，从而利用生物合成法高效生产阴蒂藻（CLT）合成的银纳米粒子（AgNPs）。方法：确定了影响因变量（即紫外可见吸光度、粒度、ZETA电位和多分散指数（PDI））的两个重要因素，即 NaOH 浓度、RH 浓度和温度作为自变量。使用透析膜和鸡蛋膜分别对制备的 CLT-AgNPs 凝胶和市场上销售的凝胶进行了体外和体内研究。此外，还对细菌菌株进行了抗菌研究。研究结果发现优化配方的粒度（114 nm）、PDI（0.45）和 ZETA 电位（-29.5 mV）分别达到了预期的水平。结果发现，制备的 CLT-AgNPs 凝胶能刺激成纤维细胞和粒细胞的发育，从而更好、更及时地促进伤口愈合。结论制备的 CLT-AgNPs 凝胶可作为一种潜在的替代品，用于急性和慢性伤口的管理和治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Drug Development and Industrial Pharmacy 医学-药学

CiteScore

6.80

自引率

0.00%

发文量

审稿时长

4.5 months

期刊介绍： The aim of Drug Development and Industrial Pharmacy is to publish novel, original, peer-reviewed research manuscripts within relevant topics and research methods related to pharmaceutical research and development, and industrial pharmacy. Research papers must be hypothesis driven and emphasize innovative breakthrough topics in pharmaceutics and drug delivery. The journal will also consider timely critical review papers.