Preliminary X-ray Diffraction Analysis of Purine Nucleoside Phosphorylase from the Haloalkaliphilic Bacterium Halomonas chromatireducens

IF 0.6 4区材料科学 Q4 CRYSTALLOGRAPHY

Crystallography Reports Pub Date : 2024-02-05 DOI:10.1134/s106377452360093x

T. N. Safonova, A. N. Antipov, V. P. Veiko, N. N. Mordkovich, N. A. Okorokova, P. V. Dorovatovskii, K. M. Polyakov

引用次数: 0

Abstract

Crystals of the enzyme purine nucleoside phosphorylase from the extremophilic bacterium Halomonas Chromatireducens AGD 8-3, suitable for X-ray diffraction, were grown by the vapor-diffusion method. The X-ray diffraction data were collected from these crystals at the Belok beamline of the Kurchatov synchrotron radiation source (National Research Centre “Kurchatov Institute”) at 100 K to 1.80 Å resolution. The X-ray diffraction data were processed in the space groups P1, P2, P2₁, and P622. The structure was solved by the molecular replacement method taking into account the twinning in the space groups P2₁ and P1 with one and two hexamers of the enzyme per asymmetric unit, respectively.

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卤代嗜碱细菌 Halomonas chromatireducens 的嘌呤核苷磷酸酶的初步 X 射线衍射分析

摘要通过蒸发扩散法从嗜极细菌 Halomonas Chromatireducens AGD 8-3 中生长出适合 X 射线衍射的嘌呤核苷磷酸化酶晶体。这些晶体的 X 射线衍射数据是在库尔恰托夫同步辐射源（国家研究中心 "库尔恰托夫研究所"）的 Belok 光束线以 100 K 的分辨率 1.80 Å 采集的。X 射线衍射数据在空间群 P1、P2、P21 和 P622 中进行了处理。考虑到 P21 和 P1 空间群中的孪生结构，每个不对称单元分别有一个和两个酶的六聚体，因此采用分子置换法对该结构进行了求解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crystallography Reports 化学-晶体学

CiteScore

1.10

自引率

28.60%

发文量

审稿时长

4-8 weeks

期刊介绍： Crystallography Reports is a journal that publishes original articles short communications, and reviews on various aspects of crystallography: diffraction and scattering of X-rays, electrons, and neutrons, determination of crystal structure of inorganic and organic substances, including proteins and other biological substances; UV-VIS and IR spectroscopy; growth, imperfect structure and physical properties of crystals; thin films, liquid crystals, nanomaterials, partially disordered systems, and the methods of studies.

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