The dwarf neon rainbowfish Melanotaenia praecox, a small spiny-rayed fish with potential as a new Acanthomorpha model fish: II. Establishment of a microinjection procedure for genetic engineering

IF 2 3区 生物学 Q2 ANATOMY & MORPHOLOGY
Kazuhide Miyamoto, Gembu Abe, Koichi Kawakami, Koji Tamura, Satoshi Ansai
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引用次数: 0

Abstract

Background

Rainbowfish is a clade of colorful freshwater fish. Melanotaenia praecox is a small rainbowfish species with biological characteristics that make it potentially useful as an experimental model species. We anticipate that M. praecox could become a new model used in various fields, such as ecology, evolution, and developmental biology. However, few previous studies have described experimental set-ups needed to understand the molecular and genetic mechanisms within this species.

Results

We describe detailed procedures for genetic engineering in the rainbowfish M. praecox. By using these procedures, we successfully demonstrated CRISPR/Cas-mediated knockout and Tol2 transposon-mediated transgenesis in this species. Regarding the CRISPR/Cas system, we disrupted the tyrosinase gene and then showed that injected embryos lacked pigmentation over much of their body. We also demonstrated that a Tol2 construct, including a GFP gene driven by a ubiquitous promoter, was efficiently integrated into the genome of M. praecox embryos.

Conclusions

The establishment of procedures for genetic engineering in M. praecox enables investigation of the genetic mechanisms behind a broad range of biological phenomena in this species. Thus, we suggest that M. praecox can be used as a new model species in various experimental biology fields.

Abstract Image

侏儒霓虹鱼(Melanotaenia praecox)--一种有可能成为新的 Acanthomorpha 模式鱼的小型刺辐鱼:II.建立基因工程显微注射程序
彩虹鱼是多彩淡水鱼的一个支系。虹彩鱼(Melanotaenia praecox)是一种小型虹彩鱼,其生物学特性使其有可能成为实验模型鱼种。我们预计,M. praecox 可能成为生态学、进化论和发育生物学等多个领域使用的新模型。然而,以前的研究很少描述了解该物种的分子和遗传机制所需的实验装置。
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来源期刊
Developmental Dynamics
Developmental Dynamics 生物-发育生物学
CiteScore
5.10
自引率
8.00%
发文量
116
审稿时长
3-8 weeks
期刊介绍: Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.
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