Micelle formation of sodium taurolithocholate

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry and Physics of Lipids Pub Date : 2024-02-05 DOI:10.1016/j.chemphyslip.2024.105378

Keisuke Matsuoka , Rina Sekiguchi , Tomokazu Yoshimura

{"title":"Micelle formation of sodium taurolithocholate","authors":"Keisuke Matsuoka , Rina Sekiguchi , Tomokazu Yoshimura","doi":"10.1016/j.chemphyslip.2024.105378","DOIUrl":null,"url":null,"abstract":"<div><p>The proportion of sodium taurolithocholate (NaTLC) is extremely low in human bile salts. NaTLC forms aggregates with other lipids in the bile and functions as an emulsifying and solubilizing agent. The molecular structure of NaTLC contains hydrophilic hydroxyl and sulfonic acid groups at both ends of the steroid ring. This molecular structure is similar to bolaform amphiphilic substance having hydrophilic groups at both ends due to the characteristics of its molecular structure. This study investigated the aggregate properties of the NaTLC using surface tension measurements, light scattering, small-angle X-ray scattering (SAXS), and cryo-transmission electron microscopy (cryo-TEM). Surface tension measurement showed that the surface tension of the NaTLC solution decreased to 54 mN m<sup>−1</sup>. The concentration that showed the minimum surface tension corresponded to the critical micelle concentration (CMC: 0.6 mmol L<sup>−1</sup>, 308 K) determined by the change in light scattering intensity. On the other hand, the degree of counterion (sodium ions) binding to the micelles increased with increasing NaTLC concentration. SAXS and cryo-TEM measurements showed that the NaTLC formed large string-like micelles. The surface activity and large aggregates showed the potential for use as biosurfactants. However, because of the relatively low solubility of NaTLC in water, its use as a biosurfactant is limited to a narrow concentration range.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemistry and Physics of Lipids","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0009308424000033","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The proportion of sodium taurolithocholate (NaTLC) is extremely low in human bile salts. NaTLC forms aggregates with other lipids in the bile and functions as an emulsifying and solubilizing agent. The molecular structure of NaTLC contains hydrophilic hydroxyl and sulfonic acid groups at both ends of the steroid ring. This molecular structure is similar to bolaform amphiphilic substance having hydrophilic groups at both ends due to the characteristics of its molecular structure. This study investigated the aggregate properties of the NaTLC using surface tension measurements, light scattering, small-angle X-ray scattering (SAXS), and cryo-transmission electron microscopy (cryo-TEM). Surface tension measurement showed that the surface tension of the NaTLC solution decreased to 54 mN m⁻¹. The concentration that showed the minimum surface tension corresponded to the critical micelle concentration (CMC: 0.6 mmol L⁻¹, 308 K) determined by the change in light scattering intensity. On the other hand, the degree of counterion (sodium ions) binding to the micelles increased with increasing NaTLC concentration. SAXS and cryo-TEM measurements showed that the NaTLC formed large string-like micelles. The surface activity and large aggregates showed the potential for use as biosurfactants. However, because of the relatively low solubility of NaTLC in water, its use as a biosurfactant is limited to a narrow concentration range.

Abstract Image

查看原文本刊更多论文

牛磺胆酸钠的胶束形成

牛磺胆酸钠（NaTLC）在人体胆汁盐中的比例极低。NaTLC 会与胆汁中的其他脂质形成聚集体，起到乳化和增溶剂的作用。NaTLC 的分子结构在类固醇环的两端含有亲水性羟基和磺酸基。由于其分子结构的特点，这种分子结构类似于两端都有亲水基团的双亲型物质（bolaform amphiphilic substance）。本研究利用表面张力测量、光散射、小角 X 射线散射（SAXS）和冷冻透射电子显微镜（cryo-TEM）研究了 NaTLC 的聚集特性。表面张力测量结果表明，NaTLC 溶液的表面张力降至 54 mN m-1。表面张力最小的浓度与根据光散射强度变化确定的临界胶束浓度（CMC：0.6 mmol L-1，308 K）相对应。另一方面，反离子（钠离子）与胶束的结合程度随着 NaTLC 浓度的增加而增加。SAXS 和 Cryo-TEM 测量显示，NaTLC 形成了大的串状胶束。其表面活性和大型聚集体显示了用作生物表面活性剂的潜力。然而，由于 NaTLC 在水中的溶解度相对较低，因此其作为生物表面活性剂的应用仅限于较窄的浓度范围。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.