The existence of senescent cells in conjunctival epithelium from elderly individuals.

IF 2.1 3区医学 Q2 OPHTHALMOLOGY

Japanese Journal of Ophthalmology Pub Date : 2024-03-01 Epub Date: 2024-02-05 DOI:10.1007/s10384-023-01047-x

Yasufumi Tomioka, Koji Kitazawa, Kohsaku Numa, Jun-Wei B Hughes, Norihiko Yokoi, Chie Sotozono

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引用次数: 0

Abstract

Purpose: The ocular surface microenvironment changes with aging. However, it remains unclear if cellular senescence influences the ocular surface. We investigated the presence of p16^INK4a-expressing senescent cells in healthy human conjunctiva.

Study design: Clinical and experimental.

Methods: Healthy conjunctival tissue samples were obtained from middle-aged and elderly subjects. RT-qPCR was performed to assess the expression of senescence markers CDKN2A (p16^INK4a) and CDKN1A (p21^CIP1/WAF1) and immunostaining was performed to examine the expression of the senescence marker p16^INK4a, stem cell markers Ki67 and p63, tight-junction marker ZO-1.

Results: Our study involved 19 conjunctival tissue samples (10 elderly and 9 middle-aged), mean age [elderly: 75.8 ± 3.7 years (72-81), middle-aged: 52.7 ± 7 years (38-59)], sex (elderly: 3 men, 7 women; middle-aged: 3 men, 6 women). The expression of p16^INK4a was significantly increased at the RNA level in the elderly compared to middle-aged (p < 0.05). Positivity rate of p16^INK4a was significantly elevated in the elderly (15.0 ± 7.8%) compared to middle-aged (0.2 ± 0.6%) (p < 0.05). Positivity rate of Ki67and p63 was significantly reduced in the elderly (1.7 ± 1.7% and 16.5 ± 9.5%) compared to middle-aged (3.9 ± 1.8% and 24.7 ± 5.7%) (p < 0.05). ZO-1 expression was reduced in tissue samples showing p16^INK4a-positivity but retained in tissue samples in which p16^INK4a was undetectable.

Conclusions: Senescent cells accumulate with age in the conjunctival epithelium, accompanied by a decrease in Ki67, p63 and ZO-1 expressing cells.

Abstract Image

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老年人结膜上皮细胞中存在衰老细胞。

目的：眼表微环境会随着衰老而发生变化。然而，细胞衰老是否会影响眼表仍不清楚。我们研究了健康人结膜中是否存在表达 p16INK4a 的衰老细胞：方法：从中老年受试者中获取健康结膜组织样本。对衰老标记物 CDKN2A（p16INK4a）和 CDKN1A（p21CIP1/WAF1）的表达进行 RT-qPCR 评估，对衰老标记物 p16INK4a、干细胞标记物 Ki67 和 p63、紧密连接标记物 ZO-1 的表达进行免疫染色检查：我们的研究涉及 19 个结膜组织样本（10 个老年样本和 9 个中年样本），平均年龄[老年：75.8 ± 3.7 岁（72-81 岁），中年：52.7 ± 7 岁（38-59 岁）]，性别（老年：3 名男性，7 名女性；中年：3 名男性，6 名女性）。与中年人相比，老年人的 p16INK4a 在 RNA 水平上的表达明显增加（p < 0.05）。老年人（15.0 ± 7.8%）的 p16INK4a 阳性率明显高于中年人（0.2 ± 0.6%）（p < 0.05）。与中年（3.9±1.8%和24.7±5.7%）相比，老年人（1.7±1.7%和16.5±9.5%）的Ki67和p63阳性率明显降低（p < 0.05）。在显示 p16INK4a 阳性的组织样本中，ZO-1 的表达量减少，但在检测不到 p16INK4a 的组织样本中，ZO-1 的表达量仍保持不变：结论：随着年龄的增长，结膜上皮中的衰老细胞会不断积累，同时Ki67、p63和ZO-1表达细胞也会减少。

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来源期刊

Japanese Journal of Ophthalmology 医学-眼科学

CiteScore

4.80

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Japanese Journal of Ophthalmology (JJO) was inaugurated in 1957 as a quarterly journal published in English by the Ophthalmology Department of the University of Tokyo, with the aim of disseminating the achievements of Japanese ophthalmologists worldwide. JJO remains the only Japanese ophthalmology journal published in English. In 1997, the Japanese Ophthalmological Society assumed the responsibility for publishing the Japanese Journal of Ophthalmology as its official English-language publication. Currently the journal is published bimonthly and accepts papers from authors worldwide. JJO has become an international interdisciplinary forum for the publication of basic science and clinical research papers.