Microbubbles activated by low-frequency ultrasound enhance the anti-tumor effects of curcumin in glioma cells by suppressing the TGF-β1/Smad/VEGF/NCAM signaling pathway.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-01-01 DOI:10.4149/gpb_2023024

Lixia Mei, Zhen Zhang, Xiaole Song, Xiaodi Zhao

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引用次数: 0

Abstract

This study investigated whether microbubbles activated by low-frequency ultrasound enhanced the anti-tumor effects of curcumin in glioma cells. CCK8 proliferation assay, scratch migration assay, and transwell invasion assay were performed to estimate the proliferation, migration, and invasion rates of the glioma cells in blank control and different treatment groups, respectively. Quantitative RT-PCR (qRT-PCR) analysis was performed to determine the relative expression levels of VEGF and NCAM mRNAs in the various experimental groups. Western blotting was performed to determine the activity status of the TGF-β1/Smad signaling pathway in various groups of glioma cells by estimating the expression levels of p-SMAD2/3, VEGF, and NCAM proteins. Combined treatment (Cur-Us-MBs) with microbubbles activated by low-frequency ultrasound and curcumin significantly reduced the in vitro proliferation, migration, and invasiveness of glioma cells compared to the control and other treatment groups. Furthermore, Cur-Us-MBs significantly reduced the expression levels of VEGF and NCAM mRNAs and proteins and p-Smad2/3 proteins , including those cells stimulated with rhTGF-β. These suggested that microbubbles activated by low-frequency ultrasound enhanced the inhibition of TGF-β1/Smad/VEGF/NCAM signaling pathway by curcumin，and enhanced the antitumor effects of curcumin by significantly reducing in vitro proliferation, migration, and invasiveness of glioma cells through this pathway.

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低频超声激活的微气泡通过抑制 TGF-β1/Smad/VEGF/NCAM 信号通路增强姜黄素在胶质瘤细胞中的抗肿瘤作用。

本研究探讨了低频超声激活的微气泡是否能增强姜黄素对胶质瘤细胞的抗肿瘤作用。研究采用 CCK8 增殖试验、划痕迁移试验和 transwell 侵袭试验，分别测定空白对照组和不同处理组胶质瘤细胞的增殖率、迁移率和侵袭率。进行定量 RT-PCR （qRT-PCR）分析，以确定不同实验组中 VEGF 和 NCAM mRNA 的相对表达水平。通过评估 p-SMAD2/3、血管内皮生长因子和 NCAM 蛋白的表达水平，进行 Western 印迹分析以确定各组胶质瘤细胞中 TGF-β1/Smad 信号通路的活性状态。与对照组和其他处理组相比，由低频超声激活的微气泡和姜黄素联合处理（Cur-Us-MBs）可显著降低胶质瘤细胞的体外增殖、迁移和侵袭性。此外，Cur-Us-MBs 还能显著降低血管内皮生长因子（VEGF）、NCAM mRNA 和蛋白以及 p-Smad2/3 蛋白的表达水平，包括受 rhTGF-β 刺激的细胞。这些结果表明，低频超声激活的微泡增强了姜黄素对TGF-β1/Smad/VEGF/NCAM信号通路的抑制作用，并增强了姜黄素的抗肿瘤作用，通过这一通路显著降低了胶质瘤细胞的体外增殖、迁移和侵袭性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.