Application of d cryo-plate technique for the cryopreservation of in vitro-grown shoot tips of Tarenaya spinosa (Cleomaceae).

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-11-01

A F R M Vilardo, G D Silva Lima, L S Cordeiro, N Albarello, C Simoes-Gurgel

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引用次数: 0

Abstract

Background: Tarenaya spinosa is a medicinal species used for treating respiratory and inflammatory diseases. Various biotechnological studies have been developed for in vitro establishment of plants and long-term conservation of this species.

Objective: This study aimed to establish a new cryopreservation protocol using the D cryo-plate technique for in vitro-grown shoot tips of T. spinosa.

Materials and methods: Different steps of the cryopreservation procedure were evaluated in this work: preculture; sucrose concentration of calcium alginate gel; concentration and time of exposure to osmoprotective loading solution; time of exposure to silica gel; and regrowth on recovery medium.

Results: The optimal procedure included preculture with increasing sucrose concentration (from 0.25 to 0.50 M), encapsulation and dehydration over silica-gel for 60 min. Increasing sucrose concentration in the loading solution or exposure duration had a negative effect on recovery of cryopreserved shoot tips. However, the association of calcium alginate gel enriched with 0.6 M sucrose with post-rewarming culture with BAP for 2 weeks resulted in the most efficient cryopreservation protocol (76% survival and 70% shoot recovery).

Conclusion: The plants developed after cryopreservation maintained their in vitro multiplication capacity and demonstrated the efficiency of long-term conservation by D cryo-plate technique for T. spinosa. Doi.org/10.54680/fr23610110512.

本刊更多论文

应用低温板技术低温冷藏 Tarenaya spinosa（铁线莲科）离体生长的嫩枝尖。

背景：刺五加是一种用于治疗呼吸道和炎症疾病的药用物种。为体外培植植物和长期保存该物种，已开展了各种生物技术研究：本研究旨在利用 D 型低温板技术，为体外培植的刺五加嫩枝尖制定新的低温保存方案：这项工作对低温保存程序的不同步骤进行了评估：预培养；海藻酸钙凝胶的蔗糖浓度；渗透保护装载溶液的浓度和暴露时间；暴露于硅胶的时间；以及在恢复培养基上的再生：最佳程序包括：增加蔗糖浓度（从 0.25 M 到 0.50 M）的预培养、封装和在硅胶上脱水 60 分钟。增加装载溶液中的蔗糖浓度或暴露时间对冷冻保存的嫩枝尖的恢复有负面影响。然而，将富含 0.6 M 蔗糖的海藻酸钙凝胶与 BAP 升温后培养 2 周的方法结合在一起，是最有效的低温保存方案（存活率为 76%，嫩枝恢复率为 70%）：结论：冷冻保存后培育出的植株保持了体外繁殖能力，证明了用D冷冻板技术长期保存刺桐的效率。Doi.org/10.54680/fr23610110512.

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.