Phœnix dactylifera, L. seed oil alleviates Bleomycin-induced pulmonary fibrosis and oxidative stress in Wistar rats.

IF 2 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biomarkers Pub Date : 2024-03-01 Epub Date: 2024-02-05 DOI:10.1080/1354750X.2024.2311178

Sana Bahri, Raed Abdennabi, Asma Chaker, Afef Nahdi, Amine Elgheryeni, Mona Mlika, Saloua Jameleddine

{"title":"Phœnix dactylifera, L. seed oil alleviates Bleomycin-induced pulmonary fibrosis and oxidative stress in Wistar rats.","authors":"Sana Bahri, Raed Abdennabi, Asma Chaker, Afef Nahdi, Amine Elgheryeni, Mona Mlika, Saloua Jameleddine","doi":"10.1080/1354750X.2024.2311178","DOIUrl":null,"url":null,"abstract":"Objective: Idiopathic pulmonary fibrosis (IPF) is the most serious form of interstitial lung disease. We aimed to investigate the effect of Phœnix dactylifera, L. seed oil (DSO) on a murine model of IPF induced by bleomycin (BLM).Methods: Male Wistar rats were treated with a single intra-tracheal injection of BLM (4 mg/kg) and a daily intraperitoneal injection of DSO (75, 150 and 300 mg/kg) for 4 weeks.Results: Our phytochemical results showed that DSO has an important antioxidant activity with a high content of polyphenols and flavonoids. High-Performance Liquid Chromatography (HPLC) and Gas chromatography/mass spectrometry (GC-MS) analysis revealed a high amount of oleic and lauric acids and a large quantity of vitamins. Histological examination showed a significant reduction in fibrosis score and collagen bands in the group of rats treated with 75 mg/kg of DSO compared to the BLM group. DSO (75 mg/kg) reversed also the increase in catalase and malondialdehyde (MDA) levels while higher doses (150 and 300 mg/kg) are ineffective against the deleterious effects of BLM. We revealed also that DSO has no renal or hepatic cytotoxic effects.Conclusion: DSO can play antioxidant and antifibrotic effects on rat models of pulmonary fibrosis at the lowest dose administered.","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"45-54"},"PeriodicalIF":2.0000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomarkers","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/1354750X.2024.2311178","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/5 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: Idiopathic pulmonary fibrosis (IPF) is the most serious form of interstitial lung disease. We aimed to investigate the effect of Phœnix dactylifera, L. seed oil (DSO) on a murine model of IPF induced by bleomycin (BLM).

Methods: Male Wistar rats were treated with a single intra-tracheal injection of BLM (4 mg/kg) and a daily intraperitoneal injection of DSO (75, 150 and 300 mg/kg) for 4 weeks.

Results: Our phytochemical results showed that DSO has an important antioxidant activity with a high content of polyphenols and flavonoids. High-Performance Liquid Chromatography (HPLC) and Gas chromatography/mass spectrometry (GC-MS) analysis revealed a high amount of oleic and lauric acids and a large quantity of vitamins. Histological examination showed a significant reduction in fibrosis score and collagen bands in the group of rats treated with 75 mg/kg of DSO compared to the BLM group. DSO (75 mg/kg) reversed also the increase in catalase and malondialdehyde (MDA) levels while higher doses (150 and 300 mg/kg) are ineffective against the deleterious effects of BLM. We revealed also that DSO has no renal or hepatic cytotoxic effects.

Conclusion: DSO can play antioxidant and antifibrotic effects on rat models of pulmonary fibrosis at the lowest dose administered.

查看原文本刊更多论文

Phœnix dactylifera, L. 种子油能减轻博莱霉素诱导的 Wistar 大鼠肺纤维化和氧化应激。

目的：特发性肺纤维化（IPF特发性肺纤维化（IPF）是最严重的间质性肺病。我们旨在研究鞘氨醇种子油（DSO）对博来霉素（BLM）诱导的 IPF 小鼠模型的影响：雄性 Wistar 大鼠气管内注射一次博来霉素（4 毫克/千克），每天腹腔注射 DSO（75、150 和 300 毫克/千克），连续 4 周：我们的植物化学研究结果表明，DSO 具有重要的抗氧化活性，其中含有大量多酚和黄酮类化合物。高效液相色谱法（HPLC）和气相色谱/质谱法（GC-MS）分析表明，DSO 含有大量油酸和月桂酸以及大量维生素。组织学检查显示，与 BLM 组相比，接受 75 毫克/千克 DSO 治疗的大鼠组的纤维化评分和胶原带明显减少。DSO（75 毫克/千克）还能逆转过氧化氢酶和丙二醛（MDA）水平的升高，而更高剂量（150 毫克和 300 毫克/千克）则对 BLM 的有害影响无效。我们还发现，DSO 对肾脏和肝脏没有细胞毒性作用：结论：在最低剂量下，DSO 对肺纤维化大鼠模型具有抗氧化和抗纤维化作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomarkers 医学-毒理学

CiteScore

5.00

自引率

3.80%

发文量

140

审稿时长

3 months

期刊介绍： The journal Biomarkers brings together all aspects of the rapidly growing field of biomarker research, encompassing their various uses and applications in one essential source. Biomarkers provides a vital forum for the exchange of ideas and concepts in all areas of biomarker research. High quality papers in four main areas are accepted and manuscripts describing novel biomarkers and their subsequent validation are especially encouraged: • Biomarkers of disease • Biomarkers of exposure • Biomarkers of response • Biomarkers of susceptibility Manuscripts can describe biomarkers measured in humans or other animals in vivo or in vitro. Biomarkers will consider publishing negative data from studies of biomarkers of susceptibility in human populations.