Diet xylo-oligosaccharide supplementation improves growth performance, immune function, and intestinal health of broilers

IF 6.1 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Nutrition Pub Date : 2024-02-03 DOI:10.1016/j.aninu.2024.01.004

Zhiyong Rao, Yue Li, Xiaopeng Yang, Yongpeng Guo, Wei Zhang, Zhixiang Wang

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Abstract

The effects of xylo-oligosaccharides (XOS) on broiler growth performance, immune function, and intestinal health were investigated. A total of 540 one-day-old Arbor Acres Plus broilers were randomly divided into 5 groups with 6 replicates per group and 18 chickens per replicate. Broilers in the control (CON) group received a corn-soybean meal based basal diet, those in the antibiotics (ANT) group received the basal diet plus 500 mg/kg oxytetracycline, and those in XOS groups received the basal diet plus 150, 300, and 450 mg/kg XOS, respectively. Compared with CON, the body weight at 42 d and average daily gain from 1 to 42 d were significantly increased in the 150, 450 mg/kg XOS-added and ANT groups (P = 0.018), and the relative expression of claudin-1 and ZO-1 mRNA in the ileum was significantly higher in the 300 and 450 mg/kg XOS-added groups (P < 0.001). The feed conversion ratios (P < 0.001) and abdominal fat rates (possibly related to short-chain fatty acids [butyric acid] produced by XOS in broilers) (P = 0.012) of broilers from 1 to 42 d of age were significantly lower in all XOS-added groups than in the control group. Splenic index (P = 0.036) and bursa index (P = 0.009) were significantly better in the ANT group and each XOS-added group than in the control group. Compared to CON and ANT, serum IgA (P = 0.007) and IgG (P = 0.002) levels were significantly higher in the 300 mg/kg XOS-added group, and the relative abundance of short-chain fatty acid-producing genera (Alistipes) was also significantly higher (P < 0.001). Meanwhile, ileal villus height (P < 0.001) and V:C (P = 0.001) were significantly increased in XOS-added broilers. In analysis of relationships between cecal microbes and the physical barrier of the gut, [Ruminococcus]_torques_group was positively correlated with mRNA expression of ileal claudin-1 (P < 0.05), and Bacteroides was positively correlated with increased ileal villus height and V:C (P < 0.05). Overall, XOS addition to broiler diets improved growth performance, promoted intestinal health by enhancing intestinal barrier function, and regulating cecal microbiota diversity, and had positive effects on immunity.

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补充低聚木糖日粮可提高肉鸡的生长性能、免疫功能和肠道健康水平

研究了木寡糖（XOS）对肉鸡生长性能、免疫功能和肠道健康的影响。将 540 只一天龄的 Arbor Acres Plus 肉鸡随机分为 5 组，每组 6 个重复，每个重复 18 只鸡。对照（CON）组的肉鸡以玉米-大豆粉为基础日粮，抗生素（ANT）组的肉鸡在基础日粮中添加 500 毫克/千克土霉素，XOS 组的肉鸡在基础日粮中分别添加 150、300 和 450 毫克/千克 XOS。与对照组相比，添加150、450 mg/kg XOS组和ANT组42 d的体重和1-42 d的平均日增重显著增加（P = 0.018），添加300和450 mg/kg XOS组回肠中claudin-1和ZO-1 mRNA的相对表达量显著增加（P < 0.001）。添加 XOS 的所有组别在 1 至 42 日龄期间的饲料转化率（P < 0.001）和腹脂率（可能与肉鸡体内 XOS 产生的短链脂肪酸 [丁酸] 有关）（P = 0.012）均显著低于对照组。ANT组和各添加XOS组的脾指数（P = 0.036）和法氏囊指数（P = 0.009）均明显优于对照组。与 CON 组和 ANT 组相比，添加 300 mg/kg XOS 组的血清 IgA（P = 0.007）和 IgG（P = 0.002）水平明显升高，短链脂肪酸生成属（Alistipes）的相对丰度也明显升高（P < 0.001）。同时，添加 XOS 的肉鸡的回肠绒毛高度（P < 0.001）和 V:C （P = 0.001）也明显增加。在分析盲肠微生物与肠道物理屏障之间的关系时，[反刍球菌]_torques_group 与回肠 claudin-1 的 mRNA 表达呈正相关（P < 0.05），而 Bacteroides 与回肠绒毛高度和 V:C 的增加呈正相关（P < 0.05）。总之，在肉鸡日粮中添加 XOS 可提高生长性能，通过增强肠道屏障功能和调节盲肠微生物群多样性来促进肠道健康，并对免疫产生积极影响。

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来源期刊

Animal Nutrition Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

7.40

自引率

3.20%

发文量

172

审稿时长

12 weeks

期刊介绍： Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to nutrition, and more applied aspects of animal nutrition, such as raw material evaluation, feed additives, nutritive value of novel ingredients and feed safety.