Molecular identification of immature Cerambycinae (Coleoptera: Cerambycidae): revisiting the significance of immature morphologies

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Seunghyun Lee, Seunghwan Lee
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引用次数: 0

Abstract

The microstructure of immature insect forms plays a crucial role in systematics, especially in inferring phylogenetic relationships and designating the morphological basis for taxonomic units. However, the microstructure of longhorn beetle larvae and pupae is poorly understood, owing to difficulties in obtaining them and technical limitations. Among 90 larvae and 80 pupae collected and photographed, 31 larvae and 15 pupae remained unidentified after morphological and bionomical evidence-based identification. Cytochrome c oxidase I sequences were amplified successfully from 68 larvae and 59 pupae. Molecular identification using tree-based analysis identified 28 of 31 undetermined larvae (90.3%) and 14 of 15 undetermined pupae (93.3%) to the species level. We highlight the usefulness of DNA barcoding in identifying immature forms of cerambycid beetles and filling gaps in public databases. This study also demonstrates how microstructure can clarify the morphological basis of polyphyletic taxa on the phylogeny and emphasizes the significance of the microstructure of immature stages in longhorn beetle systematics.
未成熟角闪虫(鞘翅目:角闪虫科)的分子鉴定:重新审视未成熟形态的意义
未成熟昆虫的微观结构在系统学中起着至关重要的作用,特别是在推断系统发育关系和指定分类单元的形态基础方面。然而,由于获取困难和技术限制,人们对长角金龟子幼虫和蛹的微观结构了解甚少。在采集并拍照的 90 只幼虫和 80 只蛹中,有 31 只幼虫和 15 只蛹在经过形态学和仿生学鉴定后仍未确定。从 68 只幼虫和 59 只蛹中成功扩增出细胞色素 c 氧化酶 I 序列。通过树状分析进行分子鉴定,31 只未确定种属的幼虫中有 28 只(90.3%)和 15 只未确定种属的蛹中有 14 只(93.3%)被确定为种属。我们强调了 DNA 条形码在鉴定未成熟形态的陶瓷甲虫和填补公共数据库空白方面的作用。本研究还展示了微观结构如何在系统发育中阐明多型类群的形态基础,并强调了未成熟阶段的微观结构在长角金龟子系统学中的重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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