Metal Release and Cytotoxicity of Different Orthodontic Bracket-Wire Combinations: An In Vitro Study.

IF 1.4 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Gunaseelan Aiswareya, Sanjeev K Verma, Saba Khan, Mohammad Owais, Izharul H Farooqi, Sufia Naseem
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引用次数: 0

Abstract

Aim: To quantify and compare the metal ions released from different bracket-wire combinations and to assess their cytotoxicity.

Materials and methods: A total of 360 fabricated sectional fixed orthodontic appliances were divided into 6 groups. The first three groups consisted of stainless-steel brackets with stainless-steel, snickel-titanium (NiTi), and titanium-molybdenum alloy (TMA) archwires, and the other three groups were fabricated using ceramic brackets (polycrystalline alumina) with stainless-steel, NiTi, and TMA archwires. These appliances were immersed in artificial saliva (pH 6.5 ± 0.5, 37°C), for 1 week, 2 weeks, and 1 month. The nickel and chromium ions released in the artificial saliva were quantified using a flame atomic absorption spectrometer, and cytotoxicity assessment was performed using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay on human cervical cancer cell line.

Results: The stainless-steel bracket groups displayed a significantly greater release of nickel and chromium ions compared to the ceramic bracket groups (P < 0.05). However, no significant differences were identified when comparing the three archwire types within the stainless-steel/ceramic bracket groups. At the end of 1 month, the % cell viability demonstrated by the appliances was in the decreasing order of stainless-steel-TMA > ceramic-stainless steel > stainless-steel-NiTi > ceramic-NiTi > stainless-steel-stainless steel > ceramic-TMA.

Conclusion: Considerably greater release of nickel and chromium ions was observed from the appliances utilizing stainless-steel brackets compared to those employing ceramic brackets. However, no remarkable difference in the levels of nickel and chromium ions was observed when comparing the three archwires: stainless steel, NiTi, and TMA. In the cytotoxicity assessment, the ceramic-TMA combination displayed the highest level of cytotoxicity, while the stainless-steel-TMA combination displayed the least cytotoxicity.

不同正畸托槽-钢丝组合的金属释放和细胞毒性:体外研究
目的:定量比较不同托槽钢丝组合释放的金属离子,并评估其细胞毒性:将 360 个制作好的分段固定正畸装置分为 6 组。前三组由不锈钢托槽与不锈钢、镍钛(NiTi)和钛钼合金(TMA)弓丝组成,另外三组使用陶瓷托槽(多晶氧化铝)与不锈钢、镍钛和 TMA 弓丝组成。这些矫治器分别浸泡在人工唾液(pH 值为 6.5 ± 0.5,37°C)中 1 周、2 周和 1 个月。使用火焰原子吸收光谱仪对人工唾液中释放的镍和铬离子进行定量,并使用 3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四氮唑(MTT)对人宫颈癌细胞株进行细胞毒性评估:不锈钢牙套组的镍和铬离子释放量明显高于陶瓷牙套组(P < 0.05)。然而,比较不锈钢/陶瓷托槽组中的三种弓丝类型,并没有发现明显的差异。1 个月结束时,矫治器显示的细胞存活率依次为不锈钢-TMA > 陶瓷-不锈钢 > 不锈钢-镍钛 > 陶瓷-镍钛 > 不锈钢-不锈钢 > 陶瓷-TMA:结论:与使用陶瓷托架的器具相比,使用不锈钢托架的器具释放出的镍和铬离子要多得多。不过,在比较不锈钢、镍钛和 TMA 这三种弓丝时,镍和铬离子的含量没有明显差异。在细胞毒性评估中,陶瓷-TMA 组合的细胞毒性最高,而不锈钢-TMA 组合的细胞毒性最低。
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来源期刊
CiteScore
2.50
自引率
0.00%
发文量
123
期刊介绍: It is a journal aimed for research, scientific facts and details covering all specialties of dentistry with a good determination for exploring and sharing the knowledge in the medical and dental fraternity. The scope is therefore huge covering almost all streams of dentistry - starting from original studies, systematic reviews, narrative reviews, very unique case reports. Journal scope is not limited to these subjects and is more wider covering all specialities of dentistry follows: -Preventive and Community dentistry (Dental public health)- Endodontics- Oral and maxillofacial pathology- Oral and maxillofacial radiology- Oral and maxillofacial surgery (also called oral surgery)- Orthodontics and dentofacial orthopedics- Periodontology (also called periodontics)- Pediatric dentistry (also called pedodontics)- Prosthodontics (also called prosthetic dentistry)- Oral medicine- Special needs dentistry (also called special care dentistry)- Oral Biology- Forensic odontology- Geriatric dentistry or Geriodontics- Preventive and Social Medicine (Public health)- Our journal appreciates research articles pertaining with advancement of dentistry, preventive and community dentistry including oral epidemiology, oral health services research, oral health education and promotion, behavioral sciences related to dentistry, dental jurisprudence, ethics and oral health, economics, and quality assessment, recent advances in preventive dentistry and community dentistry.
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