Immunomodulatory effect of Lacticaseibacillus casei CB054 supplementation in calves vaccinated against infectious bovine rhinotracheitis

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2024-02-02 DOI:10.1016/j.vetimm.2024.110726

Carolina Litchina Brasil , Vitória Sequeira Gonçalves , Francisco Denis Souza Santos , Paulo Ricardo Centeno Rodrigues , Fábio Pereira Leivas Leite , Daniela Isabel Brayer Pereira

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Abstract

Probiotics are live microorganisms that, confer health benefits to the host when supplemented in adequate amounts. They can promote immunomodulation by inducing phagocyte activity, leukocyte proliferation, antibody production, and cytokine expression. Lactic acid bacteria (BAL) are important probiotic specimens with properties that can improves ruminant nutrition, productivity and immunity. The aim of the present study was to evaluate the immunomodulatory effect of the supplementation with Lacticaseibacillus casei CB054 in calve vaccinated against bovine infectious rhinotracheitis (IBR). Calve were vaccinated with a commercial IBR vaccine, on day 0 and received a booster dose on day 21. L. casei CB054 was orally administered (4 ×10⁹ UFC) for 35 days, while a non-supplemented control group received Phosphate Buffer Saline (PBS). Stimulation of bovine splenocytes with L. casei CB054 markedly enhanced mRNA transcription levels of cytokines IL2, IL4, IL10 and IL17 genes. Calves supplemented with L. casei CB054 showed significantly higher (p < 0.05) specific anti-BoHV-1 IgG levels, higher serum neutralization, as well as higher mRNA transcription for IL2, IL4, IL10 and IL17 genes in Peripheral Blood Mononuclear Cells (PBMCs) comparing with control calves. Supplemented calve had an average weight gain of ∼14 kg more than non-supplemented during the experimental period. These results suggest that L. casei CB054 supplementation increase immunogenicity of a commercial IBR vaccine in cattle and improve weight gain.

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在接种传染性牛鼻气管炎疫苗的犊牛体内补充乳酸杆菌 CB054 对免疫调节的影响

益生菌是活的微生物，补充足量的益生菌可为宿主带来健康益处。它们可以通过诱导吞噬细胞活性、白细胞增殖、抗体产生和细胞因子表达来促进免疫调节。乳酸菌（BAL）是重要的益生菌标本，其特性可改善反刍动物的营养、生产力和免疫力。本研究旨在评估在接种牛传染性鼻气管炎（IBR）疫苗的犊牛体内补充 CB054 型乳酸杆菌对免疫调节的影响。小牛在第 0 天接种了商业 IBR 疫苗，并在第 21 天接受了加强剂量。连续 35 天口服干酪乳杆菌 CB054（4×109 UFC），而未添加干酪乳杆菌的对照组则使用磷酸盐缓冲盐水（PBS）。用 L. casei CB054 刺激牛脾细胞可显著提高细胞因子 IL2、IL4、IL10 和 IL17 基因的 mRNA 转录水平。与对照组犊牛相比，补充 L. casei CB054 的犊牛特异性抗 BoHV-1 IgG 水平明显更高（p<0.05），血清中和能力更强，外周血单核细胞（PBMCs）中 IL2、IL4、IL10 和 IL17 基因的 mRNA 转录水平也更高。在实验期间，添加辅食的犊牛比未添加辅食的犊牛平均增重约 14 千克。这些结果表明，补充 L. casei CB054 可提高牛 IBR 商业疫苗的免疫原性并提高增重。

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.