3D printable hydrogel filament with functionalizable moiety for in-situ flow-based sensor

IF 2.8 4区工程技术 Q2 POLYMER SCIENCE

Macromolecular Research Pub Date : 2024-02-02 DOI:10.1007/s13233-023-00238-2

Kwangmo Go, Dong-Myung Kim, Kyung Jin Lee

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引用次数: 0

Abstract

Understanding microorganisms is a worthy work to gather various biological information that can directly affect human beings. However, most information is detected or measured at ex-situ conditions. In this work, we attempted to fabricate a 3D printable hydrogel-based in-situ detection system. We designed the hydrogel containing azide functionalized polyethylenglycole methacrylate (PEGMA) and fabricated the hydrogel as a 3D structure to prepare flow type detector using a 3D printer. To use hydrogel as a 3D printer filament, we enhanced viscosity via the pre-crosslinking process and added bentonite with Polyethylene glycol diacrylate (PEGDA) crosslinker with a certain proportion. Prepared hydrogel 3D structure could cultivate E.coli in a liquid culture medium. The hydrogel 3D structure has an azide group which is a useful tool to introduce additional chemical functionality via azide–alkyne click reaction. Using this process, we introduced alkyne functionalized 4-(2-pyridylazo) resorcinol (PAR). The PAR clicked hydrogel can be used as flow based sensor platform to detect i.e. various metal ions including Cu, Al, and Co ions in media.

Graphical Abstract

Schematic diagram of final concept of azide-functionalized hydrogel-based in-situ detector

Abstract Image

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带有可功能化分子的可三维打印水凝胶丝，用于现场流量传感器

了解微生物是一项很有价值的工作，可以收集到直接影响人类的各种生物信息。然而，大多数信息都是在原位条件下检测或测量的。在这项工作中，我们尝试制造一种基于三维打印水凝胶的原位检测系统。我们设计了含有叠氮功能化聚乙二醇甲基丙烯酸酯（PEGMA）的水凝胶，并使用三维打印机将水凝胶制作成三维结构，以制备流动型检测器。为了将水凝胶用作三维打印机丝，我们通过预交联工艺提高了粘度，并在聚乙二醇二丙烯酸酯（PEGDA）交联剂中添加了一定比例的膨润土。制备的水凝胶三维结构可以在液体培养基中培养大肠杆菌。水凝胶三维结构具有叠氮基团，可以通过叠氮-炔烃点击反应引入额外的化学功能。利用这一工艺，我们引入了炔官能化的 4-（2-吡啶偶氮）间苯二酚（PAR）。PAR 点击水凝胶可用作基于流动的传感器平台，用于检测介质中的各种金属离子，包括铜、铝和钴离子。

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来源期刊

Macromolecular Research 工程技术-高分子科学

CiteScore

4.70

自引率

8.30%

发文量

100

审稿时长

1.3 months

期刊介绍： Original research on all aspects of polymer science, engineering and technology, including nanotechnology Presents original research articles on all aspects of polymer science, engineering and technology Coverage extends to such topics as nanotechnology, biotechnology and information technology The English-language journal of the Polymer Society of Korea Macromolecular Research is a scientific journal published monthly by the Polymer Society of Korea. Macromolecular Research publishes original researches on all aspects of polymer science, engineering, and technology as well as new emerging technologies using polymeric materials including nanotechnology, biotechnology, and information technology in forms of Articles, Communications, Notes, Reviews, and Feature articles.