Effects of particle size, storage conditions, and chemical pretreatments on carbon and oxygen isotopic measurements of modern tooth enamel

IF 1.9 4区 医学 Q2 MEDICINE, LEGAL
Francesca Spencer , Kirsten Verostick , Alejandro Serna , Chris Stantis , Gabriel J. Bowen
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引用次数: 0

Abstract

Isotopic analysis of human tooth enamel can provide life history information useful in forensic identification. These applications depend on the availability of reference data documenting isotopic values for individuals with known life history and on the comparability of data from reference and case work samples. Here we build on previous methodological research, which has largely focused on paleontological and archaeological samples, and conduct experiments using enamel from modern human teeth targeting three sample preparation variables (sample particle size, storage conditions, and chemical pretreatments). Our results suggest that differences in particle size affect the efficiency of sample reactions during pretreatment and analysis, with coarse particles giving reduced loss of enamel carbonate during acid pretreatments but producing slightly higher oxygen isotope values than fine particles during analysis. Data for samples stored in dry and ambient environments following pretreatment were indistinguishable, suggesting no exchange of oxygen between samples and ambient water vapor. Finally, chemical pretreatments with a range of commonly used reactants and conditions showed a pervasive, moderate oxygen isotope shift associated with acetic acid treatment, which may be caused by exchange of enamel hydroxyl groups with reagents or rinse waters. Collectively, the results emphasize the importance of methodological standardization to improve comparability and reduce potential for bias in the forensic application of tooth enamel isotope data.

粒度、储存条件和化学预处理对现代牙釉质碳和氧同位素测量的影响
人类牙釉质的同位素分析可以提供有助于法医鉴定的生活史信息。这些应用取决于是否有参考数据记录已知生活史个体的同位素值,以及参考样本和案例样本数据的可比性。以前的方法学研究主要集中在古生物学和考古学样本上,我们在此基础上利用现代人牙齿的珐琅质进行实验,针对三个样本制备变量(样本粒度、储存条件和化学预处理)进行研究。我们的结果表明,颗粒大小的不同会影响样品在预处理和分析过程中的反应效率,粗颗粒在酸预处理过程中会减少珐琅质碳酸盐的损失,但在分析过程中产生的氧同位素值却略高于细颗粒。预处理后保存在干燥环境和环境中的样本数据没有区别,表明样本与环境水蒸气之间没有氧气交换。最后,在一系列常用反应物和条件下进行的化学预处理显示,与醋酸处理相关的氧同位素偏移普遍、适度,这可能是珐琅质羟基与试剂或冲洗水发生交换造成的。总之,这些结果强调了方法标准化的重要性,以提高可比性并减少牙釉质同位素数据在法医应用中可能出现的偏差。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Science & Justice
Science & Justice 医学-病理学
CiteScore
4.20
自引率
15.80%
发文量
98
审稿时长
81 days
期刊介绍: Science & Justice provides a forum to promote communication and publication of original articles, reviews and correspondence on subjects that spark debates within the Forensic Science Community and the criminal justice sector. The journal provides a medium whereby all aspects of applying science to legal proceedings can be debated and progressed. Science & Justice is published six times a year, and will be of interest primarily to practising forensic scientists and their colleagues in related fields. It is chiefly concerned with the publication of formal scientific papers, in keeping with its international learned status, but will not accept any article describing experimentation on animals which does not meet strict ethical standards. Promote communication and informed debate within the Forensic Science Community and the criminal justice sector. To promote the publication of learned and original research findings from all areas of the forensic sciences and by so doing to advance the profession. To promote the publication of case based material by way of case reviews. To promote the publication of conference proceedings which are of interest to the forensic science community. To provide a medium whereby all aspects of applying science to legal proceedings can be debated and progressed. To appeal to all those with an interest in the forensic sciences.
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