Comparison of PET tracing and biodistribution between 64Cu-labeled micro-and nano-polystyrene in a murine inhalation model

IF 7.2 1区医学 Q1 TOXICOLOGY

Particle and Fibre Toxicology Pub Date : 2024-01-31 DOI:10.1186/s12989-023-00561-7

Joycie Shanmugiah, Javeria Zaheer, Changkeun Im, Choong Mo Kang, Jin Su Kim

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引用次数: 0

Abstract

Recent studies showed the presence of microplastic in human lungs. There remains an unmet need to identify the biodistribution of microplastic after inhalation. In this study, we traced the biodistribution of inhaled micro-sized polystyrene (mPS) and/or nano-sized PS (nPS) using 64Cu with PET in mice. We used 0.2–0.3-µm sized mPS and 20-nm sized nPS throughout. 64Cu-DOTA-mPS, 64Cu-DOTA-nPS and/or 64CuCl2 were used to trace the distribution in the murine inhalation model. PET images were acquired using an INVEON PET scanner at 1, 12, 24, 48, and 72 h after intratracheal instillation, and the SUVmax for interesting organs were determined, biodistribution was then determined in terms of percentage injected dose/gram of tissue (%ID/g). Ex vivo tissue-radio thin-layer chromatography (Ex vivo-radioTLC) was used to demonstrate the existence of 64Cu-DOTA-PS in tissue. PET image demonstrated that the amount of 64Cu-DOTA-mPS retained within the lung was significantly higher than 64Cu-DOTA-nPS until 72 h; SUVmax values of 64Cu-DOTA-mPS in lungs was 11.7 ± 5.0, 48.3 ± 6.2, 65.5 ± 2.3, 42.2 ± 13.1, and 13.2 ± 2.3 at 1, 12, 24, 48, and 72 h respectively whereas it was 31.2 ± 3.1, 17.3 ± 5.9, 10.0 ± 3.4, 8.1 ± 2.4 and 8.9 ± 3.6 for 64Cu-DOTA-nPS at the corresponding timepoints. The biodistribution data supported the PET data with a similar pattern of clearance of the radioactivity from the lung. nPS cleared rapidly post instillation in comparison to mPS within the lungs. Higher accumulation of %ID/g for nPS (roughly 2 times) were observed compared to mPS in spleen, liver, intestine, thymus, kidney, brain, salivary gland, ovary, and urinary bladder. Ex vivo-radioTLC was used to demonstrate that the detected gamma rays originated from 64Cu-DOTA-mPS or nPS. PET image demonstrated the differences in accumulations of mPS and/or nPS between lungs and other interesting organs. The information provided may be used as the basis for future studies on the toxicity of mPS and/or nPS.

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在小鼠吸入模型中比较 64Cu 标记的微聚苯乙烯和纳米聚苯乙烯的 PET 追踪和生物分布情况

最近的研究表明，人体肺部存在微塑料。确定微塑料吸入后的生物分布仍然是一个尚未满足的需求。在这项研究中，我们利用 64Cu 和 PET 对小鼠吸入的微尺寸聚苯乙烯（mPS）和/或纳米尺寸聚苯乙烯（nPS）的生物分布进行了追踪。我们使用了 0.2-0.3-µm 尺寸的 mPS 和 20-nm 尺寸的 nPS。我们使用 64Cu-DOTA-mPS、64Cu-DOTA-nPS 和/或 64CuCl2 来追踪小鼠吸入模型中的分布情况。使用 INVEON PET 扫描仪在气管内灌注后 1、12、24、48 和 72 小时采集 PET 图像，确定相关器官的 SUVmax，然后以注射剂量/克组织百分比（%ID/g）确定生物分布。利用体内组织-放射薄层色谱法（Ex vivo-radioTLC）证明了 64Cu-DOTA-PS 在组织中的存在。PET 图像显示，在 72 h 之前，64Cu-DOTA-mPS 在肺部的保留量明显高于 64Cu-DOTA-nPS；64Cu-DOTA-mPS 在肺部的 SUVmax 值分别为 11.7 ± 5.0、48.3 ± 6.2、65.而 64Cu-DOTA-nPS 在相应时间点的 SUVmax 值分别为 31.2 ± 3.1、17.3 ± 5.9、10.0 ± 3.4、8.1 ± 2.4 和 8.9 ± 3.6。与 mPS 相比，nPS 在灌入肺部后会迅速清除。与 mPS 相比，nPS 在脾脏、肝脏、肠道、胸腺、肾脏、大脑、唾液腺、卵巢和膀胱中的累积 %ID/g 值更高（约为 mPS 的 2 倍）。利用活体放射层析技术证明检测到的伽马射线来自 64Cu-DOTA-mPS 或 nPS。PET 图像显示了肺部和其他相关器官中 mPS 和/或 nPS 累积量的差异。所提供的信息可作为今后研究 mPS 和/或 nPS 毒性的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Particle and Fibre Toxicology TOXICOLOGY-

CiteScore

15.90

自引率

4.00%

发文量

审稿时长

6 months

期刊介绍： Particle and Fibre Toxicology is an online journal that is open access and peer-reviewed. It covers a range of disciplines such as material science, biomaterials, and nanomedicine, focusing on the toxicological effects of particles and fibres. The journal serves as a platform for scientific debate and communication among toxicologists and scientists from different fields who work with particle and fibre materials. The main objective of the journal is to deepen our understanding of the physico-chemical properties of particles, their potential for human exposure, and the resulting biological effects. It also addresses regulatory issues related to particle exposure in workplaces and the general environment. Moreover, the journal recognizes that there are various situations where particles can pose a toxicological threat, such as the use of old materials in new applications or the introduction of new materials altogether. By encompassing all these disciplines, Particle and Fibre Toxicology provides a comprehensive source for research in this field.