Selective neural inhibition via photobiomodulation alleviates behavioral hypersensitivity associated with small sensory fiber activation

IF 2.2 3区医学 Q2 DERMATOLOGY

Lasers in Surgery and Medicine Pub Date : 2024-01-30 DOI:10.1002/lsm.23762

Andrew Buzza MSc, Kalista Tapas BS, Junqi Zhuo PhD, Juanita J. Anders PhD, Stephen J. Lewis PhD, Michael W. Jenkins PhD, Michael Moffitt PhD

{"title":"Selective neural inhibition via photobiomodulation alleviates behavioral hypersensitivity associated with small sensory fiber activation","authors":"Andrew Buzza MSc, Kalista Tapas BS, Junqi Zhuo PhD, Juanita J. Anders PhD, Stephen J. Lewis PhD, Michael W. Jenkins PhD, Michael Moffitt PhD","doi":"10.1002/lsm.23762","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>Photobiomodulation at higher irradiances has great potential as a pain-alleviating method that selectively inhibits small diameter nerve fibers and corresponding sensory experiences, such as nociception and heat sensation. The longevity and magnitude of these effects as a function of laser irradiation parameters at the nerve was explored.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>In a rodent chronic pain model (spared nerve injury—SNI), light was applied directly at the sural nerve with four delivery schemes: two irradiance levels (7.64 and 2.55 W/cm<sup>2</sup>) for two durations each, corresponding to either 4.8 or 14.4 J total energy, and the effect on sensory hypersensitivities was evaluated.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>At emitter irradiances of 7.64 W/cm<sup>2</sup> (for 240 s), 2.55 W/cm<sup>2</sup> (for 720 s), and 7.64 W/cm<sup>2</sup> (for 80 s) the heat hypersensitivity was relieved the day following photobiomodulation (PBM) treatment by 37 ± 8.1% (statistically significant, <i>p</i> < 0.001), 26% ± 6% (<i>p</i> = 0.072), and 28 ± 6.1% (statistically significant, <i>p</i> = 0.032), respectively, and all three treatments reduced the hypersensitivity over the course of the experiment (13 days) at a statistically significant level (mixed-design analysis of variance, <i>p</i> < 0.05). The increases in tissue temperature (5.3 ± 1.0 and 1.3 ± 0.4°C from 33.3°C for the higher and lower power densities, respectively) at the neural target were well below those typically associated with permanent action potential disruption.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>The data from this study support the use of direct PBM on nerves of interest to reduce sensitivities associated with small-diameter fiber activity.</p>\n </section>\n </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"56 3","pages":"305-314"},"PeriodicalIF":2.2000,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lsm.23762","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lasers in Surgery and Medicine","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/lsm.23762","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DERMATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

Photobiomodulation at higher irradiances has great potential as a pain-alleviating method that selectively inhibits small diameter nerve fibers and corresponding sensory experiences, such as nociception and heat sensation. The longevity and magnitude of these effects as a function of laser irradiation parameters at the nerve was explored.

Methods

In a rodent chronic pain model (spared nerve injury—SNI), light was applied directly at the sural nerve with four delivery schemes: two irradiance levels (7.64 and 2.55 W/cm²) for two durations each, corresponding to either 4.8 or 14.4 J total energy, and the effect on sensory hypersensitivities was evaluated.

Results

At emitter irradiances of 7.64 W/cm² (for 240 s), 2.55 W/cm² (for 720 s), and 7.64 W/cm² (for 80 s) the heat hypersensitivity was relieved the day following photobiomodulation (PBM) treatment by 37 ± 8.1% (statistically significant, p < 0.001), 26% ± 6% (p = 0.072), and 28 ± 6.1% (statistically significant, p = 0.032), respectively, and all three treatments reduced the hypersensitivity over the course of the experiment (13 days) at a statistically significant level (mixed-design analysis of variance, p < 0.05). The increases in tissue temperature (5.3 ± 1.0 and 1.3 ± 0.4°C from 33.3°C for the higher and lower power densities, respectively) at the neural target were well below those typically associated with permanent action potential disruption.

Conclusions

The data from this study support the use of direct PBM on nerves of interest to reduce sensitivities associated with small-diameter fiber activity.

Abstract Image

查看原文本刊更多论文

通过光生物调节进行选择性神经抑制，可减轻与小感觉纤维激活相关的行为超敏性。

目的：较高辐照度下的光生物调节作为一种缓解疼痛的方法具有巨大潜力，可选择性地抑制小直径神经纤维和相应的感觉体验，如痛觉和热觉。本研究探讨了这些效应的持续时间和程度与激光照射神经的参数之间的关系：方法：在啮齿类慢性疼痛模型（裸神经损伤-SNI）中，用四种传输方案将光直接照射到鞍神经上：两种辐照度水平（7.64 和 2.55 W/cm2），每种持续时间为两个，对应的总能量为 4.8 或 14.4 J：结果：在发射器辐照度分别为 7.64 W/cm2（240 秒）、2.55 W/cm2（720 秒）和 7.64 W/cm2（80 秒）时，光生物调制（PBM）治疗后第二天的热超敏反应缓解了 37 ± 8.1%（有统计学意义，P 结论）：本研究的数据支持在相关神经上使用直接光生物调控来降低与小直径纤维活动相关的敏感性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Lasers in Surgery and Medicine 医学-外科

CiteScore

5.40

自引率

12.50%

发文量

119

审稿时长

1 months

期刊介绍： Lasers in Surgery and Medicine publishes the highest quality research and clinical manuscripts in areas relating to the use of lasers in medicine and biology. The journal publishes basic and clinical studies on the therapeutic and diagnostic use of lasers in all the surgical and medical specialties. Contributions regarding clinical trials, new therapeutic techniques or instrumentation, laser biophysics and bioengineering, photobiology and photochemistry, outcomes research, cost-effectiveness, and other aspects of biomedicine are welcome. Using a process of rigorous yet rapid review of submitted manuscripts, findings of high scientific and medical interest are published with a minimum delay.