Transcriptomic analysis of the submandibular gland under psychological stress condition

IF 2.7 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine Pub Date : 2024-01-30 DOI:10.1111/jop.13512

Durga Paudel, Osamu Uehara, Sarita Giri, Tetsuro Morikawa, Koki Yoshida, Takao Kitagawa, Dedy Ariwansa, Nisha Acharya, Kazunori Ninomiya, Yasuhiro Kuramitsu, Tohru Ohta, Masanobu Kobayashi, Yoshihiro Abiko

{"title":"Transcriptomic analysis of the submandibular gland under psychological stress condition","authors":"Durga Paudel, Osamu Uehara, Sarita Giri, Tetsuro Morikawa, Koki Yoshida, Takao Kitagawa, Dedy Ariwansa, Nisha Acharya, Kazunori Ninomiya, Yasuhiro Kuramitsu, Tohru Ohta, Masanobu Kobayashi, Yoshihiro Abiko","doi":"10.1111/jop.13512","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Psychological stress is associated with changes in salivary flow and composition. However, studies to show the effect of psychological stress on the transcriptome of the salivary gland are limited. This study aims to perform a transcriptomic analysis of the submandibular gland under psychological stress using a chronic restraint stress model of rats.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Sprague-Dawley rats were divided into stress groups and control groups. Psychological stress was induced in the stress group rats by enclosing them in a plastic tube for 4 h daily over 6 weeks. RNA sequencing was performed on RNA extracted from the submandibular gland. The differentially expressed genes were identified, and the genes of interest were further validated using qRT-PCR, immunofluorescence, and western blot.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>A comparison between control and stress groups showed 45 differentially expressed genes. The top five altered genes in RNA sequencing data showed similar gene expression in qRT-PCR validation. The most downregulated gene in the stress group, <i>FosB</i>, was a gene of interest and was further validated for its protein-level expression using immunofluorescence and western blot. The genesets for gene ontology cellular component, molecular function, and KEGG showed that pathways related to ribosome biosynthesis and function were downregulated in the stress group compared to the control.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Psychological stress showed transcriptomic alteration in the submandibular gland. The findings may be important in understanding stress-related oral diseases.</p>\n </section>\n </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"53 2","pages":"150-158"},"PeriodicalIF":2.7000,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral Pathology & Medicine","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jop.13512","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Psychological stress is associated with changes in salivary flow and composition. However, studies to show the effect of psychological stress on the transcriptome of the salivary gland are limited. This study aims to perform a transcriptomic analysis of the submandibular gland under psychological stress using a chronic restraint stress model of rats.

Methods

Sprague-Dawley rats were divided into stress groups and control groups. Psychological stress was induced in the stress group rats by enclosing them in a plastic tube for 4 h daily over 6 weeks. RNA sequencing was performed on RNA extracted from the submandibular gland. The differentially expressed genes were identified, and the genes of interest were further validated using qRT-PCR, immunofluorescence, and western blot.

Results

A comparison between control and stress groups showed 45 differentially expressed genes. The top five altered genes in RNA sequencing data showed similar gene expression in qRT-PCR validation. The most downregulated gene in the stress group, FosB, was a gene of interest and was further validated for its protein-level expression using immunofluorescence and western blot. The genesets for gene ontology cellular component, molecular function, and KEGG showed that pathways related to ribosome biosynthesis and function were downregulated in the stress group compared to the control.

Conclusion

Psychological stress showed transcriptomic alteration in the submandibular gland. The findings may be important in understanding stress-related oral diseases.

查看原文本刊更多论文

心理压力条件下的下颌下腺转录组分析

背景：心理压力与唾液流量和成分的变化有关。然而，显示心理压力对唾液腺转录组影响的研究却很有限。本研究旨在利用大鼠慢性束缚应激模型，对心理应激下的颌下腺进行转录组分析：方法：将 Sprague-Dawley 大鼠分为应激组和对照组。方法：将 Sprague-Dawley 大鼠分为应激组和对照组，将应激组大鼠关在塑料管中，每天 4 小时，持续 6 周，诱导大鼠产生心理应激。对从颌下腺提取的 RNA 进行 RNA 测序。对差异表达的基因进行了鉴定，并使用 qRT-PCR、免疫荧光和 Western 印迹对感兴趣的基因进行了进一步验证：结果：对照组和应激组之间的比较显示出 45 个差异表达基因。RNA 测序数据中变化最大的五个基因在 qRT-PCR 验证中显示出相似的基因表达。应激组中下调幅度最大的基因 FosB 是一个值得关注的基因，并通过免疫荧光和 Western 印迹进一步验证了其蛋白质水平的表达。基因本体细胞成分、分子功能和 KEGG 的基因组显示，与对照组相比，应激组与核糖体生物合成和功能相关的通路下调：结论：心理压力会改变下颌下腺的转录组。结论：心理压力会改变下颌下腺的转录组学，这些发现可能对了解与压力相关的口腔疾病有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Oral Pathology & Medicine 医学-病理学

CiteScore

5.90

自引率

6.10%

发文量

121

审稿时长

4-8 weeks

期刊介绍： The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.