New indole derivatives from endophytic fungus Colletotruchum sp. HK-08 originated from leaves of Nerium indicum

IF 4.7 4区医学 Q1 CHEMISTRY, MEDICINAL

Chinese Herbal Medicines Pub Date : 2024-04-01 DOI:10.1016/j.chmed.2023.07.004

Huiqin Chen , Hao Zheng , Caihong Cai , Hao Wang , Cuijuan Gai , Zhiqiong Tan , Haofu Dai , Wenli Mei

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引用次数: 0

Abstract

Objective

To study secondary metabolites from endophytic fungus Colletotruchum sp. HK-08 originated from the leaves of Nerium indicum.

Methods

The compounds were isolated by various column chromatographic techniques, and their structures were elucidated by spectroscopic techniques [high resolution electrospray ionization mass spectroscopy (HRESIMS), one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance spectroscopy (NMR)], as well as comparison with literature data. The Ellman method was used to determine the acetylcholinesterase (AChE) inhibitory activity.

Results

Four indole derivatives were identified from Colletotruchum sp. HK-08, including 6′-hydroxymonaspiloindole (1), 2-(2-oxoindolin-3-yl)ethyl 2-(4-hydroxyphenyl) acetate (2), 2-(2-oxoindolin-3-yl)ethyl 2-(2-hydroxyphenyl)acetate (3), and monaspiloindole (4). Compound 4 presented weak AChE inhibitory activity with IC₅₀ value of (69.30 ± 6.27) μmol/L [tacrine as the positive control, with IC₅₀ value of (0.61 ± 0.07) μmol/L].

Conclusion

Compounds 1–3 were new compounds, and compound 4 had weak AChE inhibitory activity.

查看原文本刊更多论文

源自 Nerium indicum 叶子的内生真菌 Colletotruchum sp.

方法采用不同的柱层析技术分离化合物，通过光谱技术[高分辨率电喷雾离子化质谱（HRESIMS）、一维（1D）和二维（2D）核磁共振波谱（NMR）]以及与文献数据的比较阐明其结构。结果从 Colletotruchum sp.HK-08 中鉴定出四种吲哚衍生物，包括 6′-羟基单天冬氨醛（1）、2-(2-氧代吲哚啉-3-基)乙基 2-(4-羟基苯基)乙酸酯（2）、2-(2-氧代吲哚啉-3-基)乙基 2-(2-羟基苯基)乙酸酯（3）和单天冬氨醛（4）。化合物 4 具有较弱的 AChE 抑制活性，IC50 值为 (69.30 ± 6.27) μmol/L [他克林作为阳性对照，IC50 值为 (0.61 ± 0.07) μmol/L]。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chinese Herbal Medicines CHEMISTRY, MEDICINAL-

CiteScore

4.40

自引率

5.30%

发文量

629

审稿时长

10 weeks

期刊介绍： Chinese Herbal Medicines is intended to disseminate the latest developments and research progress in traditional and herbal medical sciences to researchers, practitioners, academics and administrators worldwide in the field of traditional and herbal medicines. The journal's international coverage ensures that research and progress from all regions of the world are widely included. CHM is a core journal of Chinese science and technology. The journal entered into the ESCI database in 2017, and then was included in PMC, Scopus and other important international search systems. In 2019, CHM was successfully selected for the “China Science and Technology Journal Excellence Action Plan” project, which has markedly improved its international influence and industry popularity. CHM obtained the first impact factor of 3.8 in Journal Citation Reports (JCR) in 2023.