Screening of Antimicrobial Producing Bacteria from Berawe Beach Sand on Kampai Pangkalan Susu Island against Pathogenic Bacteria

Rafika Annisa, Kartika Manalu, Rizki Amelia Nasution
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Abstract

Sand is naturally a place to live, grow and develop some marine animals and microorganisms, usually bacteria originating from the sea area that have a large number of bioactive compounds that can produce various kinds of secondary metabolites for further production of antimicrobials. The purpose of this study was to obtain bacteria that have the potential to produce antimicrobials and to characterize bacterial isolates on the sand of Berawe Beach, Kampai Island, Pangkalan Susu. In this study several stages were carried out, namely sampling, isolation, purification, morphological characterization, antimicrobial activity test, gram staining, biochemical test and determining the bacterial genus. The results of this study obtained 9 isolates (SP14A. SP15A, SP16A, SP24A, SP24B, SP35A, SP44A, SP44B and SP45A) which have the potential as antimicrobial producers. Characterization of antimicrobial bacterial isolates from the sand of Berawe Beach, Kampai Island, Pangkalan Susu, namely isolate SP14A, which is a gram- positive bacterium in the form of streptobacilli. SP15A, SP24B and SP44B isolates were coccus-shaped gram-positive bacteria. SP16A, SP35A, SP44A and SP45A isolates were gram-positive bacteria in the form of bacilli. And isolate SP24A is a gram-negative bacterium in the form of streptobacilli. All isolates produce catalase enzymes but do not use carbon and energy. Isolates that are motile (SP14A, SP15A, SP16A, SP24A, SP35A, SP44A and SP45A) can ferment glucose while non-motile (SP24B and SP44B) cannot ferment glucose. These bacteria come from the genera Bacillus, Micrococcus, LactoBacillus and Alcaligenes. Species of Bacillus pumilus, Bacillus subtilis, Bacillus firmus, LactoBacillus bulgaricus, Micrococcus luteus and Alcaligenes eutrophus.
坎派彭卡兰苏苏岛 Berawe 海滩沙子中产生抗菌素的细菌对病原菌的筛选
沙子是一些海洋动物和微生物生存、生长和发展的天然场所,这些微生物通常是源自海域的细菌,它们具有大量生物活性化合物,可以产生各种次级代谢物,从而进一步生产抗菌剂。本研究的目的是获取有可能产生抗菌素的细菌,并对彭卡兰苏苏(Pangkalan Susu)坎派岛贝拉韦海滩(Berawe Beach)沙子上的细菌分离物进行鉴定。这项研究分为几个阶段,即取样、分离、纯化、形态特征描述、抗菌活性测试、革兰氏染色、生化测试和确定细菌属。研究结果表明,9 个分离菌株(SP14A、SP15A、SP16A、SP24A、SP24B、SP35A、SP44A、SP44B 和 SP45A)具有抗菌潜力。从彭卡兰苏苏(Pangkalan Susu)坎拜岛(Kampai Island)贝拉韦海滩(Berawe Beach)的沙子中分离出的抗菌细菌的特征,即分离物 SP14A,它是一种链杆菌形式的革兰氏阳性细菌。SP15A、SP24B 和 SP44B 分离物为球状革兰氏阳性菌。SP16A、SP35A、SP44A 和 SP45A 分离物为杆菌形式的革兰氏阳性细菌。SP24A 分离物是链球菌形式的革兰氏阴性细菌。所有分离物都能产生过氧化氢酶,但不利用碳和能量。能运动的分离株(SP14A、SP15A、SP16A、SP24A、SP35A、SP44A 和 SP45A)能发酵葡萄糖,而不能运动的分离株(SP24B 和 SP44B)则不能发酵葡萄糖。这些细菌属于芽孢杆菌属、微球菌属、乳杆菌属和嗜钙杆菌属。其中包括枯草芽孢杆菌(Bacillus pumilus)、枯草芽孢杆菌(Bacillus subtilis)、坚固芽孢杆菌(Bacillus firmus)、保加利亚乳杆菌(LactoBacillus bulgaricus)、黄体微球菌(Micrococcus luteus)和富营养化阿卡列奈氏菌(Alcaligenes eutrophus)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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