Exploring Anti-Breast Cancer Effects of Live Pediococcus acidilactici and Its Cell-Free Supernatant Isolated from Human Breast Milk

IF 1.6 Q4 ONCOLOGY
Naa N. Adumuah, J. T. Quarshie, Harry Danwonno, A. Aikins, Elmer N. Ametefe
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引用次数: 0

Abstract

Current breast cancer treatment options are limited by drug resistance and adverse side effects, which calls for the need for alternatives or complementary remedies. Probiotic bacteria isolated from human breast milk have been shown to possess proapoptotic and anti-inflammatory properties against breast mastitis in breastfeeding mothers and are being studied as possible anticancer regimens. Thus, this study aimed at exploring the effect of lactic acid bacteria isolated from human breast milk on MDA-MB 231 breast cancer cells. A total of twenty-two bacteria were isolated from four human breast milk samples. The isolates were characterized and identified using biochemical tests and Sanger sequencing, respectively. For in vitro experiments, we used isolated P. acidilactici to treat MDA-MB-231 cells, and an MTT assay was used to detect proliferation. RT-qPCR and wound healing assays were performed to determine the effect of the isolated P. acidilactici on breast cancer cytokine expression and migration. Exposure of MDA-MB 231 breast cancer cells to live P. acidilactici and its cell-free supernatant (CFS) for 24 h resulted in a reduction in cancer cell viability. Also, the expression of the cytokines IL-6, IL-8, and IL-10 in the breast cancer cells increased following exposure to P. acidilactici and its CFS for 24 and 72 h. Additionally, the levels of the SLUG gene remained unchanged while the TWIST1 gene was upregulated following exposure of the cancer cells to bacteria, indicating that P. acidilactici may promote epithelial-mesenchymal transition in breast cancer. Finally, the CFS significantly inhibited cancer cell mobility. These findings serve as a foundation to further investigate the usefulness of P. acidilactici as a potential therapeutic agent in breast cancer therapy.
探索从人类母乳中分离出的酸乳球菌及其无细胞上清液的抗乳腺癌作用
目前的乳腺癌治疗方案受到耐药性和不良副作用的限制,因此需要替代或补充疗法。从母乳中分离出的益生菌已被证明对哺乳期母亲的乳腺炎具有促凋亡和抗炎作用,目前正被研究作为可能的抗癌疗法。因此,本研究旨在探索从母乳中分离的乳酸菌对 MDA-MB 231 乳腺癌细胞的影响。从四份母乳样本中共分离出 22 种细菌。我们分别使用生化测试和桑格测序法对分离菌进行了表征和鉴定。在体外实验中,我们用分离出的 P. acidilactici 处理 MDA-MB-231 细胞,并用 MTT 检测法检测细胞的增殖情况。为了确定分离的 P. acidilactici 对乳腺癌细胞因子表达和迁移的影响,我们进行了 RT-qPCR 和伤口愈合试验。将 MDA-MB 231 乳腺癌细胞暴露于活的 P. acidilactici 及其无细胞上清液(CFS)中 24 小时后,癌细胞存活率降低。此外,乳腺癌细胞中的细胞因子 IL-6、IL-8 和 IL-10 的表达在接触 P. acidilactici 及其 CFS 24 小时和 72 小时后有所增加。最后,CFS 能明显抑制癌细胞的移动性。这些发现为进一步研究 P. acidilactici 作为一种潜在的乳腺癌治疗药物的有用性奠定了基础。
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来源期刊
CiteScore
3.40
自引率
0.00%
发文量
25
审稿时长
19 weeks
期刊介绍: International Journal of Breast Cancer is a peer-reviewed, Open Access journal that provides a forum for scientists, clinicians, and health care professionals working in breast cancer research and management. The journal publishes original research articles, review articles, and clinical studies related to molecular pathology, genomics, genetic predisposition, screening and diagnosis, disease markers, drug sensitivity and resistance, as well as novel therapies, with a specific focus on molecular targeted agents and immune therapies.
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