alidation of an analytical method by HPLC for the quantification of yangambin from the Ocotea duckei species

IF 0.7 4区医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas Pub Date : 2024-01-30 DOI:10.37360/blacpma.24.23.1.09

L. Peixoto, J. V. Chaves Junior, F. Andrade, I. Basílio, Fabio Santos de Souza, J. Barbosa Filho

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Abstract

Ocotea duckei, known as Louro-de-cheiro, belongs to the Lauraceae family and presents lignoid yangambine (YAN) as the main plant marker. This work aimed to develop and validate an analytical method by high performance liquid chromatography for the quantification of YAN. The sample used was the crude ethanolic extract (CEE) obtained from aerial parts. In the developed method, a C18 column was used. The mobile phase was composed of acetonitrile and water (45:55), whereas the method parameters included mobile phase flow rate at 0.8 mL/min, oven temperature at 40°C, and monitoring at 205 nm. In the validation, the parameters of selectivity, linearity, precision, accuracy, robustness, limits of detection and quantification were evaluated. As a result, the developed method is in accordance with the guidelines for validation of analytical methods and presented satisfactory chromatographic parameters for YAN determination. Thus, the present analytical methodology can be applied in the quality control of O. duckei raw materials.

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用高效液相色谱法验证定量分析 Ocotea duckei 物种中洋姜素的分析方法

Ocotea duckei（又名 Louro-de-cheiro）属于月桂科植物，其主要植物标记为木质洋兰碱（YAN）。本研究旨在开发并验证一种高效液相色谱法，用于定量分析 YAN。使用的样品是从气生部分提取的粗乙醇提取物（CEE）。该方法采用C18色谱柱。流动相为乙腈-水（45:55），流速为0.8 mL/min，柱温为40 ℃，监测波长为205 nm。在验证过程中，对选择性、线性、精密度、准确度、稳健性、检出限和定量限等参数进行了评价。结果表明，所开发的方法符合分析方法验证指南的要求，在测定 YAN 时的色谱参数令人满意。因此，本分析方法可用于杜仲原料的质量控制。

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来源期刊

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas PHARMACOLOGY & PHARMACY-

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas (BLACPMA), [Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants]; currently edited by the publishing house MS-Editions, is a bi-monthly international publication that publishes original peerreviewed research in the field of medicinal and aromatic plants, with nearly 20 years of experience. BLACPMA is a scientific journal that publishes two types of articles: Reviews (only in English) and Original Articles (Spanish or English), its main lines of action being agronomy, anthropology and ethnobotany, industrial applications, botany, quality and standardization, ecology and biodiversity, pharmacology, phytochemistry, pharmacognosy, regulatory and legislative aspects. While all areas of medicinal plants are welcome and the experimental approaches used can be broad and interdisciplinary; other areas of research that are not mentioned depend on the Editorial Committee for their acceptance.