The Cytotoxicity Effect of Chitosan-Encapsulated Ricin-Herceptin Immunotoxin Nanoparticles on Breast Cancer Cell Lines

IF 1.4 4区材料科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Nanoscience Pub Date : 2024-01-29 DOI:10.2174/0115734137278545240102055626

Mohammad Hossein Golestani Poor, Shohreh Zare Karizi, Seyed Ali Mirhosseini, Mohammad Javad Motamedi, Fateme Frootan, Soghra Khani, Jafar Amani

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Abstract

Background:: The use of targeted therapy has been increasing for cancer treatment. The aim of this study is to investigate chitosan-based ricin-Herceptin (rh) immunotoxin on breast cancer cell lines. background: The targeted therapy is growing for cancer treatment. The aim here is to investigate a chitosan based Ricin-Herceptin (rh) immunotoxin on breast cancer cell lines. Methods:: The gene construct encoding immunotoxin was designed, cloned, and expressed in E. coli BL21 (DE3). The expressed proteins were isolated by the nickel-nitrilotriacetic acid column and were analyzed by the Western-blotting. The cytotoxicity of immunotoxin was assayed on breast cell line MCF-7 and using MTT assay at 24 and 48 h treatment. Results:: The immunotoxins extrication rate, size, loading percentage, and electric charge of nanoparticles were reported appropriately as 78%, 151.5 nm, 83.53%, and +11.1 mV, respectively. The encapsulated immunotoxins led to the death of 70% and 78% of MCF-7 cells at 24 and 48 h treatment, respectively. The noncapsulated counterparts at equal doses killed 53% and 62% of cancer cells at the same time points. Conclusion:: The chitosan-immunotoxins impose potential cytotoxic effects on cancer cells. other: Keywords: Herceptin, HER2, Ricin, Targeted therapy, Breast Cancer, Nanoparticles

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壳聚糖包裹的蓖麻毒素-赫赛汀免疫毒素纳米粒子对乳腺癌细胞株的细胞毒性效应

背景靶向治疗在癌症治疗中的应用越来越广泛。本研究旨在研究壳聚糖基蓖麻毒素-赫赛汀（rh）免疫毒素对乳腺癌细胞株的作用：癌症治疗中的靶向疗法越来越多。本研究旨在研究基于壳聚糖的蓖麻毒素-赫赛汀（rh）免疫毒素对乳腺癌细胞系的作用。研究方法设计、克隆并在大肠杆菌 BL21 (DE3) 中表达编码免疫毒素的基因构建体。用镍-三乙酸镍柱分离表达的蛋白质，并进行 Western 印迹分析。免疫毒素对乳腺细胞株 MCF-7 的细胞毒性检测采用 MTT 法，处理时间为 24 小时和 48 小时。结果显示纳米颗粒的免疫毒素提取率、尺寸、负载率和电荷分别为78%、151.5 nm、83.53%和+11.1 mV。在处理 24 和 48 小时后，封装的免疫毒素分别导致 70% 和 78% 的 MCF-7 细胞死亡。在相同的时间点，同等剂量的非包封免疫毒素分别杀死了 53% 和 62% 的癌细胞。结论壳聚糖免疫毒素对癌细胞具有潜在的细胞毒性作用：关键词赫赛汀、HER2、蓖麻毒素、靶向治疗、乳腺癌、纳米颗粒

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来源期刊

Current Nanoscience 工程技术-材料科学：综合

CiteScore

3.50

自引率

6.70%

发文量

审稿时长

4.4 months

期刊介绍： Current Nanoscience publishes (a) Authoritative/Mini Reviews, and (b) Original Research and Highlights written by experts covering the most recent advances in nanoscience and nanotechnology. All aspects of the field are represented including nano-structures, nano-bubbles, nano-droplets and nanofluids. Applications of nanoscience in physics, material science, chemistry, synthesis, environmental science, electronics, biomedical nanotechnology, biomedical engineering, biotechnology, medicine and pharmaceuticals are also covered. The journal is essential to all researches involved in nanoscience and its applied and fundamental areas of science, chemistry, physics, material science, engineering and medicine. Current Nanoscience also welcomes submissions on the following topics of Nanoscience and Nanotechnology: Nanoelectronics and photonics Advanced Nanomaterials Nanofabrication and measurement Nanobiotechnology and nanomedicine Nanotechnology for energy Sensors and actuator Computational nanoscience and technology.