Vascular endothelial cellular mechanics under hyperglycemia and its role in tissue regeneration

IF 5.6 1区医学 Q1 MATERIALS SCIENCE, BIOMATERIALS

Regenerative Biomaterials Pub Date : 2024-01-26 DOI:10.1093/rb/rbae004

Kui Wang, Yongmei Ge, Yongshuai Yang, Zhenjian Li, Jiayi Liu, Yizebang Xue, Yuanjun Zhang, Xiangchao Pang, A H W Ngan, Bin Tang

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引用次数: 0

Abstract

Diabetes is one of the most prevalent diseases worldwide. The tissue regeneration of diabetes patients is known to be rather tricky as the result of vascular dysfunction, and this leads to various clinical complications including diabetic foot ulcers. The vascular endothelial cells compactly line the inner surface of blood vessels are responsible for the growth and maintenance of blood vessels, and play an essential role in tissue regeneration. Although the mechanical properties of cells are generally known to be regulated by physiological/pathological conditions, few studies have been performed to investigate vascular endothelial cellular mechanics under hyperglycemia and the biological functions related to tissue regeneration. In this study, we conduct a systematic investigation of this issue. The results suggested that the stiffness of human umbilical vein endothelial cells (HUVECs) can be significantly regulated by the glucose concentration, subsequently, leading to significant alterations in cell migration and proliferation capabilities that are closely related to tissue regeneration. The rearrangement of the cytoskeleton induced by hyperglycemia through Cdc42 was found to be one of the pathways for the alteration of the cell stiffness and the subsequent cell dysfunctions. Therefore, we suggested that the inhibition of Cdc42 might be a promising strategy to facilitate various tissue regeneration for diabetes patients.

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高血糖状态下的血管内皮细胞力学及其在组织再生中的作用

糖尿病是全球最普遍的疾病之一。众所周知，由于血管功能障碍，糖尿病患者的组织再生相当棘手，这导致了包括糖尿病足溃疡在内的各种临床并发症。血管内皮细胞紧密排列在血管内表面，负责血管的生长和维护，在组织再生中发挥着至关重要的作用。虽然人们普遍知道细胞的力学特性受生理/病理条件的调控，但很少有研究探讨高血糖条件下血管内皮细胞的力学特性以及与组织再生相关的生物功能。在本研究中，我们对这一问题进行了系统的调查。结果表明，人脐静脉内皮细胞（HUVECs）的硬度可受葡萄糖浓度的显著调节，进而导致与组织再生密切相关的细胞迁移和增殖能力的显著改变。研究发现，高血糖通过 Cdc42 诱导的细胞骨架重排是改变细胞硬度和随后导致细胞功能障碍的途径之一。因此，我们认为抑制 Cdc42 可能是促进糖尿病患者各种组织再生的一种有前途的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regenerative Biomaterials Materials Science-Biomaterials

CiteScore

7.90

自引率

16.40%

发文量

审稿时长

10 weeks

期刊介绍： Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.