Swim-up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing.

IF 2.7 3区医学 Q2 OBSTETRICS & GYNECOLOGY

Reproductive Medicine and Biology Pub Date : 2024-01-29 eCollection Date: 2024-01-01 DOI:10.1002/rmb2.12562

Kenji Amano, Satoko Oigawa, Koichiro Ichizawa, Yukiko Tokuda, Mami Unagami, Mami Sekiguchi, Mayuko Furui, Kentaro Nakaoka, Ayumu Ito, Rika Hayashi, Yuko Tamaki, Yuko Hayashi, Yusuke Fukuda, Yukiko Katagiri, Masahiko Nakata, Koichi Nagao

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引用次数: 0

Abstract

Purpose: This study aimed to evaluate the effects of swim-up and density gradient centrifugation methods on sperm DNA fragmentation.

Methods: Nineteen normozoospermic patient samples with ≥100 × 10⁶ motile sperms were included in this study. Sperm DNA fragmentation, progressive motility, and progressive motile sperm number were measured before and after the swim-up method or density gradient centrifugation.

Results: Sperm DNA fragmentation was not statistically different between swim-up-(14.4 ± 2.1%, p = 0.32) and density gradient centrifugation-processed (25.0 ± 3.0%, p = 0.20) and unprocessed semen samples (19.2 ± 1.9%). Sperm DNA fragmentation was significantly lower in swim-up-than in density gradient centrifugation-processed samples (p < 0.05). Sperm progressive motility was significantly higher (p < 0.05) in swim-up-(92.9 ± 1.0%) and density gradient centrifugation-processed (81.3 ± 2.0%) samples, with the former being higher, than in unprocessed semen samples (53.1 ± 3.7%). The recovery rate of progressive motile sperms was significantly lower in swim-up-(9.7 ± 1.4%) than in density gradient centrifugation-processed samples (17.2 ± 1.8%, p < 0.05).

Conclusions: The swim-up method is superior to density gradient centrifugation, evidenced by less sperm DNA fragmentation and higher sperm progressive motility. The recovery rate of progressive motile sperms was better after density gradient centrifugation than after swim-up.

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在精子处理过程中，泳动法比密度梯度离心法更能保持精子 DNA 的完整性。

目的：本研究旨在评估游离法和密度梯度离心法对精子DNA碎片的影响：方法：本研究纳入了 19 份活动精子数≥100 × 106 的正常无精子症患者样本。在游动法或密度梯度离心前后测量精子DNA片段、活动力和活动精子数量：精子DNA片段在游动法（14.4 ± 2.1%，p = 0.32）和密度梯度离心法处理过的精液样本（25.0 ± 3.0%，p = 0.20）与未处理过的精液样本（19.2 ± 1.9%）之间没有统计学差异。泅渡法处理的精液样本的精子 DNA 碎片率明显低于密度梯度离心法处理的样本（p p p 结论：泅渡法优于密度梯度离心法：游离法优于密度梯度离心法，这体现在精子DNA碎片更少，精子的渐进运动能力更强。密度梯度离心后，渐进式运动精子的回收率优于游离法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive Medicine and Biology Multiple-

CiteScore

5.70

自引率

5.90%

发文量

审稿时长

20 weeks

期刊介绍： Reproductive Medicine and Biology (RMB) is the official English journal of the Japan Society for Reproductive Medicine, the Japan Society of Fertilization and Implantation, the Japan Society of Andrology, and publishes original research articles that report new findings or concepts in all aspects of reproductive phenomena in all kinds of mammals. Papers in any of the following fields will be considered: andrology, endocrinology, oncology, immunology, genetics, function of gonads and genital tracts, erectile dysfunction, gametogenesis, function of accessory sex organs, fertilization, embryogenesis, embryo manipulation, pregnancy, implantation, ontogenesis, infectious disease, contraception, etc.