Cryptocarya moschata extract decreases single and mixed biofilms on acrylic resins.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2024-10-01 Epub Date: 2024-01-29 DOI:10.1111/odi.14858

Camilla Olga Tasso, Beatriz Ribeiro Ribas, Túlio Morandin Ferrisse, Jonatas Silva de Oliveira, Janaina Habib Jorge

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引用次数: 0

Abstract

Objective: This study proposed to assess the effect of Cryptocarya moschata extract on single and mixed biofilms formed on denture base and reline acrylic resin.

Materials and methods: Single and mixed biofilms of Candida albicans and Streptococcus mutans were formed on the samples and treated with C. moschata extract; Nystatin solution at 100,000 IU/mL or Penicillin antibiotic solution at 100,000 IU/mL; or PBS solution. Antimicrobial activity was analyzed by counting colony-forming units, metabolism assay, assessment of protein components of the biofilm matrix, and of cell viability using confocal laser scanning microscopy (CLSM). Data were submitted to ANOVA and Tukey's post-test (α = 0.05).

Results: Cryptocarya moschata extract reduced cell viability of C. albicans and S. mutans single and mixed biofilms formed on samples. For all types of biofilms in the C. moschata group, there was a log reduction of the biofilm, proven by the Alamar Blue assay. Analyzing the extracellular matrix protein components, groups treated with the extract exhibited a lower level of fluorescence compared to the PBS groups. Reduction in thickness biofilm and viable cells was perceptible in the C. moschata group when assessing through CLSM.

Conclusion: Cryptocarya moschata extract reduced the single and mixed biofilms of C. albicans and S. mutans on acrylic resins.

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隐翅虫提取物可减少丙烯酸树脂上的单一和混合生物膜。

研究目的本研究旨在评估隐翅虫提取物对义齿基托和衬垫丙烯酸树脂上形成的单一和混合生物膜的影响：在样品上形成白色念珠菌和变异链球菌的单一生物膜和混合生物膜，并用隐翅虫提取物、100,000 IU/mL 的奈司他丁溶液或 100,000 IU/mL 的青霉素抗生素溶液或 PBS 溶液进行处理。通过菌落形成单位计数、新陈代谢测定、生物膜基质蛋白质成分评估以及使用激光共聚焦扫描显微镜（CLSM）评估细胞存活率来分析抗菌活性。数据采用方差分析和 Tukey 后检验（α = 0.05）：结果：隐鞭毛菜提取物降低了样本上形成的白僵菌和变异单胞菌单一生物膜和混合生物膜的细胞活力。阿拉玛蓝检测法证明，在 C. moschata 组的所有类型生物膜中，生物膜都减少了对数。在分析细胞外基质蛋白成分时，与 PBS 组相比，用提取物处理的组显示出较低的荧光水平。通过 CLSM 评估，C. moschata 组的生物膜厚度和存活细胞数量明显减少：结论：Cryptocarya moschata 提取物可减少丙烯酸树脂上白种人球菌和变异人球菌的单一和混合生物膜。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.