Curcumin and Berberine Arrest Maturation and Activation of Dendritic Cells Derived from Lupus Erythematosus Patients.

Amin Reza Nikpoor, Mahmoud Mahmoudi, Abbas Shapouri-Moghaddam, Zahra Rezaieyazdi, Samaneh Mollazadeh, Nafiseh Tabasi, Atena Mansouri, Reyhane Modarres Moghadam, Amir Abbas Momtazi, Soran K Najmaldin, Ramiar Kamal Kheder, Seyed-Alireza Esmaeili
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Abstract

Background: Systemic lupus erythematosus (SLE) is a complex autoimmune disease recognized by elevated activity of autoimmune cells, loss of tolerance, and decreased regulatory T cells producing inhibitory cytokines. Despite many efforts, the definitive treatment for lupus has not been fully understood. Curcumin (CUR) and berberine (BBR) have significant immunomodulatory roles and anti-inflammatory properties that have been demonstrated in various studies. This study aimed to investigate the anti-inflammatory properties of CUR and BBR on human monocyte-derived dendritic cells (DCs) with an special focus on the maturation and activation of DCs.

Methods: Human monocytes were isolated from the heparinized blood of SLE patients and healthy individuals, which were then exposed to cytokines (IL-4 and GM-CSF) for five days to produce immature DCs. Then, the obtained DCs were characterized by FITC-uptake assay and then cultured in the presence of CUR, BBR, or lipopolysaccharide (LPS) for 48 h. Finally, the maturation of DCs was analyzed by the level of maturation using flow cytometry or real-time PCR methods.

Results: The results showed promising anti-inflammatory effects of CUR and BBR in comparison with LPS, supported by a significant reduction of not only co-stimulatory and antigen-presenting factors such as CD80, CD86, CD83, CD1a, CD14, and HLA-DR but also inflammatory cytokines such as IL-12.

Conclusion: CUR and BBR could arrest DC maturation and develop a tolerogenic DC phenotype that subsequently promoted the expression of inhibitory cytokines and reduced the secretion of proinflammatory markers.

姜黄素和小檗碱能阻止红斑狼疮患者树突状细胞的成熟和活化
背景:系统性红斑狼疮(SLE)是一种复杂的自身免疫性疾病,表现为自身免疫细胞活性增强、耐受性丧失以及产生抑制性细胞因子的调节性 T 细胞减少。尽管做了很多努力,但狼疮的最终治疗方法仍未完全明了。姜黄素(CUR)和小檗碱(BBR)具有显著的免疫调节作用和抗炎特性,这已在多项研究中得到证实。方法:从系统性红斑狼疮患者和健康人肝素化血液中分离人单核细胞,然后将其暴露于细胞因子(IL-4 和 GM-CSF)中 5 天,以产生未成熟的 DCs。然后,用 FITC 摄取测定法对获得的 DCs 进行表征,再在 CUR、BBR 或脂多糖(LPS)存在下培养 48 小时:结果:结果表明,与 LPS 相比,CUR 和 BBR 具有很好的抗炎效果,不仅共刺激因子和抗原递呈因子(如 CD80、CD86、CD83、CD1a、CD14 和 HLA-DR)显著减少,而且炎性细胞因子(如 IL-12)也显著减少:结论:CUR 和 BBR 可阻止 DC 成熟并形成一种可耐受的 DC 表型,从而促进抑制性细胞因子的表达并减少促炎标志物的分泌。
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