HER2 aptamer-conjugated iron oxide nanoparticles with PDMAEMA-b-PMPC coating for breast cancer cell identification.

Nanomedicine (London, England) Pub Date : 2024-02-01 Epub Date: 2024-01-29 DOI:10.2217/nnm-2023-0225

Cyro von Zuben de Valega Negrão, Natália Np Cerize, Amauri da Silva Justo-Junior, Raquel Bester Liszbinski, Giovanna Pastore Meneguetti, Larissa Araujo, Silvana A Rocco, Kaliandra de Almeida Gonçalves, Daniel R Cornejo, Patrícia Leo, Caio Perecin, Douglas Adamoski, Sandra M Gomes Dias

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Abstract

Aim: To synthesize HER2 aptamer-conjugated iron oxide nanoparticles with a coating of poly(2-(dimethylamino) ethyl methacrylate)-poly(2-methacryloyloxyethylphosphorylcholine) block copolymer (IONPPPs). Methods: Characterization covered molecular structure, chemical composition, thermal stability, magnetic characteristics, aptamer interaction, crystalline nature and microscopic features. Subsequent investigations focused on IONPPPs for in vitro cancer cell identification. Results: Results demonstrated high biocompatibility of the diblock copolymer with no significant toxicity up to 150 μg/ml. The facile coating process yielded the IONPP complex, featuring a 13.27 nm metal core and a 3.10 nm polymer coating. Functionalized with a HER2-targeting DNA aptamer, IONPPP enhanced recognition in HER2-amplified SKBR3 cells via magnetization separation. Conclusion: These findings underscore IONPPP's potential in cancer research and clinical applications, showcasing diagnostic efficacy and HER2 protein targeting in a proof-of-concept approach.

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具有 PDMAEMA-b-PMPC 涂层的 HER2 通感素共轭氧化铁纳米粒子用于乳腺癌细胞识别。

目的：合成包覆聚(2-(二甲基氨基)乙基甲基丙烯酸酯)-聚(2-甲基丙烯酰氧乙基磷酰胆碱)嵌段共聚物（IONPPPs）的 HER2 类似物共轭氧化铁纳米粒子。方法：表征内容包括分子结构、化学成分、热稳定性、磁性特征、载体相互作用、结晶性质和显微特征。随后的研究重点是用于体外癌细胞鉴定的 IONPPPs。研究结果结果表明，二嵌段共聚物具有很高的生物相容性，在 150 μg/ml 以下无明显毒性。通过简便的包覆工艺获得了 IONPP 复合物，其特点是具有 13.27 nm 的金属内核和 3.10 nm 的聚合物包覆层。IONPPP 与 HER2 靶向 DNA 类似物功能化后，通过磁化分离增强了对 HER2 扩增的 SKBR3 细胞的识别能力。结论这些发现凸显了 IONPPP 在癌症研究和临床应用方面的潜力，展示了概念验证方法的诊断效果和 HER2 蛋白靶向性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nanomedicine (London, England)

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