Weihuan Hu, Zheng Jin, Huihua Wang, Fangfang Wang, Fan Qu
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引用次数: 0
Abstract
Phthalates (PAEs), a group of environmental endocrine disruptors, are associated with oxidative stress and have adverse effects on female ovarian reserves. However, this association has been poorly investigated, particularly with respect to clinical evidence. In this study, we provided clinical evidence of a relationship between exposure levels of PAEs, oxidative stress and decreased ovarian reserve (DOR). Firstly, the urinary concentrations of metabolites of PAEs were measured by high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). The serum concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and anti-Mullerian hormone (AMH), and the biomarkers of oxidative stress, malondialdehyde (MDA), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC), were determined. Finally, statistical analyses were conducted to describe the relationship between the PAEs exposure, oxidative stress and DOR. We found that the levels of monomethyl phthalate (MMP), monoisobutyl phthalate (MiBP), mono-(2-ethylhexyl) phthalate (MEHP), and mono-(2-ethyl-5-hydroxypentyl) phthalate (MECPP) in the DOR group were significantly higher than those in the control group. There was a significant negative association between AMH and MMP, MiBP levels. and a significant positive association between FSH and MMP levels. PAEs exposure was also associated with a significant increase in MDA levels and decrease in SOD levels. In conclusion, the exposure of PAEs was closely associated with DOR, potentially mediated by oxidative stress pathways; however, small sample size was a limitation in this study.
邻苯二甲酸盐(PAEs)是一类环境内分泌干扰物,与氧化应激有关,并对女性卵巢储备产生不利影响。然而,对这种关联性的研究却很少,特别是在临床证据方面。在这项研究中,我们提供了 PAEs 暴露水平、氧化应激和卵巢储备功能下降(DOR)之间关系的临床证据。首先,我们采用高效液相色谱-串联质谱法(HPLC-MS/MS)测定了尿液中 PAEs 代谢物的浓度。测定了血清中促卵泡激素(FSH)、黄体生成素(LH)和抗穆勒氏管激素(AMH)的浓度,以及氧化应激的生物标志物丙二醛(MDA)、超氧化物歧化酶(SOD)和总抗氧化能力(T-AOC)。最后,对 PAEs 暴露、氧化应激和 DOR 之间的关系进行了统计分析。我们发现,DOR 组中邻苯二甲酸单甲酯(MMP)、邻苯二甲酸单异丁酯(MiBP)、邻苯二甲酸单(2-乙基己基)酯(MEHP)和邻苯二甲酸单(2-乙基-5-羟基戊基)酯(MECPP)的含量明显高于对照组。AMH与MMP、MiBP水平呈明显负相关,FSH与MMP水平呈明显正相关。暴露于 PAEs 还与 MDA 水平的明显升高和 SOD 水平的降低有关。总之,暴露于 PAEs 与 DOR 密切相关,可能是由氧化应激途径介导的;然而,样本量小是本研究的一个局限。
期刊介绍:
Toxicology & Industrial Health is a journal dedicated to reporting results of basic and applied toxicological research with direct application to industrial/occupational health. Such research includes the fields of genetic and cellular toxicology and risk assessment associated with hazardous wastes and groundwater.