Impact of air pollution from different sources on sperm DNA methylation.

IF 2.2 4区医学 Q3 ENVIRONMENTAL SCIENCES

International Journal of Environmental Health Research Pub Date : 2024-10-01 Epub Date: 2024-01-28 DOI:10.1080/09603123.2024.2310152

Miluse Vozdova, Svatava Kubickova, Vera Kopecka, Alfredo Pauciullo, Jiri Rubes

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引用次数: 0

Abstract

Environmental exposure is associated with increased incidence of respiratory and cardiovascular diseases and reduced fertility. Exposure to air pollution can influence gene expression through epigenetic mechanisms. In this study, we analysed gene-specific CpG methylation in spermatozoa of city policemen occupationally exposed to air pollution in two Czech cities differing by sources and composition of the air pollution. In Prague, the pollution is mainly formed by NO2 from heavy traffic. Ostrava is a hotspot of industrial air pollution with high concentrations of particular matter (PM) and benzo[a]pyrene (B[a]P). We performed genome-wide methylation sequencing using the SureSelectXT Human Methyl-Seq system (Agilent Technologies) and next-generation sequencing to reveal differentially methylated CpG sites and regions. We identified differential methylation in the region chr5:662169 - 663376 annotated to genes CEP72 and TPPP. The region was then analysed in sperm DNA from 117 policemen using targeted methylation sequencing, which proved its hypermethylation in sperm of Ostrava policemen.

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不同来源的空气污染对精子 DNA 甲基化的影响

暴露于环境中与呼吸道和心血管疾病发病率的增加以及生育率的降低有关。暴露于空气污染可通过表观遗传机制影响基因表达。在这项研究中，我们分析了在捷克两个空气污染来源和成分不同的城市中，职业暴露于空气污染的城市警察精子中特异基因的 CpG 甲基化。在布拉格，污染主要由交通繁忙造成的二氧化氮形成。俄斯特拉发是工业空气污染的热点地区，特殊物质（PM）和苯并[a]芘（B[a]P）浓度较高。我们使用 SureSelectXT Human Methyl-Seq 系统（安捷伦科技公司）和新一代测序技术进行了全基因组甲基化测序，以揭示不同的甲基化 CpG 位点和区域。我们在注释为基因 CEP72 和 TPPP 的 chr5:662169 - 663376 区域发现了不同的甲基化。随后，我们使用靶向甲基化测序技术对 117 名警察的精子 DNA 中的该区域进行了分析，结果证明在俄斯特拉发警察的精子中该区域存在高甲基化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Environmental Health Research 医学-公共卫生、环境卫生与职业卫生

CiteScore

6.70

自引率

3.10%

发文量

134

审稿时长

>12 weeks

期刊介绍： International Journal of Environmental Health Research ( IJEHR ) is devoted to the rapid publication of research in environmental health, acting as a link between the diverse research communities and practitioners in environmental health. Published articles encompass original research papers, technical notes and review articles. IJEHR publishes articles on all aspects of the interaction between the environment and human health. This interaction can broadly be divided into three areas: the natural environment and health – health implications and monitoring of air, water and soil pollutants and pollution and health improvements and air, water and soil quality standards; the built environment and health – occupational health and safety, exposure limits, monitoring and control of pollutants in the workplace, and standards of health; and communicable diseases – disease spread, control and prevention, food hygiene and control, and health aspects of rodents and insects. IJEHR is published in association with the International Federation of Environmental Health and includes news from the Federation of international meetings, courses and environmental health issues.