Antibiotic resistance and genomic characterization of Mycobacterium abscessus complex isolates from patients with pulmonary tuberculosis in Iran: A multi-center study (2010-2021).

IF 1.3 4区医学 Q4 IMMUNOLOGY

Acta microbiologica et immunologica Hungarica Pub Date : 2024-01-29 Print Date: 2024-03-26 DOI:10.1556/030.2024.02169

Mohammad Hashemzadeh, Aram Asareh Zadegan Dezfuli, AzarDokht Khosravi, Nazanin Ahmad Khosravi, Morteza Saki

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Abstract

One hundred and eighteen sputum specimens suspected of Mycobacterium abscessus infection were collected. Species level identification of M. abscessus was performed by rpoB sequencing. Clonality analysis was done by multilocus sequence typing (MLST) for M. abscessus. Antibiotic susceptibility testing was performed for clarithromycin, amikacin, ciprofloxacin and moxifloxacin. Altogether 128 isolates were obtained and were subjected to rpoB gene sequencing for definite identification. Among them 59 were identified as M. abscessus, and these included 22 (37.28%) isolates of M. abscessus subsp. abscessus, 22 (37.28%) isolates of M. abscessus subsp. massiliense, and 15 (25.42%) isolates of M. abscessus subsp. bolletii. All 59 M. abscessus complex isolates were analyzed by MLST in this study. Certain sequence types (STs) were identified among the 59 isolates and were specific for each subspecies. Two STs (ST40 and ST33) were specific to M. abscessus subsp. abscessus, one ST (ST20) was specific to M. abscessus subsp. bolletii, and one ST (ST15) was specific to M. abscessus subsp. massiliense. In antibiotic resistance, clarithromycin susceptibility testing of 22 M. abscessus subsp. abscessus strains detected 15 (68.18%) resistant strains, while among 22 M. abscessus subsp. massiliense strains 5 (22.72%) exhibited resistance, and among 15 M. abscessus subsp. bolletii 8 (53.33%) were resistant. Our study revealed a significant level of antibiotic resistance in isolates of the M. abscessus complex.

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伊朗肺结核患者脓肿分枝杆菌复合体的抗生素耐药性和基因组特征：一项多中心研究（2010-2021 年）。

收集了 118 份疑似脓肿分枝杆菌感染的痰标本。通过 rpoB 测序对脓肿分枝杆菌进行种级鉴定。通过脓肿分枝杆菌多焦点序列分型（MLST）进行克隆性分析。对克拉霉素、阿米卡星、环丙沙星和莫西沙星进行了抗生素药敏试验。共获得 128 个分离株，并对其进行 rpoB 基因测序，以确定其身份。其中 59 个被鉴定为脓肿疽杆菌，包括 22 个（37.28%）脓肿疽杆菌亚种分离株、22 个（37.28%）脓肿疽杆菌亚种分离株和 15 个（25.42%）脓肿疽杆菌亚种分离株。本研究对所有 59 个复合脓肿病菌分离物进行了 MLST 分析。在这 59 个分离株中发现了一些序列类型（STs），它们对每个亚种都具有特异性。两个 ST（ST40 和 ST33）是脓毒血症 M. abscessus 亚种的特异性，一个 ST（ST20）是脓毒血症 M. abscessus 亚种的特异性，一个 ST（ST15）是脓毒血症 M. abscessus 亚种的特异性。在抗生素耐药性方面，对 22 株脓肿荚膜杆菌亚种进行克拉霉素敏感性检测，发现 15 株（68.18%）耐药，22 株脓肿荚膜杆菌亚种中有 5 株（22.72%）耐药，15 株脓肿荚膜杆菌亚种中有 8 株（53.33%）耐药。我们的研究显示，脓毒症蕈菌复合体分离菌株的抗生素耐药性水平很高。

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来源期刊

Acta microbiologica et immunologica Hungarica IMMUNOLOGY-MICROBIOLOGY

CiteScore

2.30

自引率

13.30%

发文量

审稿时长

>12 weeks

期刊介绍： AMIH is devoted to the publication of research in all fields of medical microbiology (bacteriology, virology, parasitology, mycology); immunology of infectious diseases and study of the microbiome related to human diseases.