Purple Carrot Extract Exhibits a Neuroprotective Profile in th e Nigrostriatal Pathway in the Reserpine-induced Model of Parkinson 's Disease.

Ana Claudia Custódio-Silva, Jose Ivo Araújo Beserra-Filho, Beatriz Soares-Silva, Amanda Maria-Macêdo, Suellen Silva-Martins, Sara Pereira Silva, José Ronaldo Santos, Regina Helena Silva, Daniel Araki Ribeiro, Alessandra Mussi Ribeiro
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Abstract

Background: Parkinson's disease (PD) is a chronic neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons in the nigrostriatal pathway. Even with scientific and technological advances, the therapeutic approaches used for the treatment of PD have shown to be largely ineffective in controlling the progression of symptoms in the long term. There is a growing demand for the development of novel therapeutic strategies for PD treatment. Different herbs and supplements have been considered as adjuvant to treat the symptoms of Parkinsonism. The carrot is one of the most consumed vegetable species worldwide, and its root is known for its content of anthocyanins, which possess antioxidant and antiinflammatory properties. This study evaluated the neuroprotective effect of purple carrot extract (CAR) in rats on the reserpine (RES)-induced progressive parkinsonism model.

Methods: Male rats (6-month-old) received orally the CAR (400 mg/kg) or vehicle and subcutaneously RES (0.01 mg/kg) or vehicle for 28 days (Preventive Phase). From the 29th day, rats received CAR or vehicle daily and RES (0.1 mg/kg) or vehicle every other day (for 23 days, Protective phase). Behavioral tests were conducted throughout the treatment. Upon completion, the animals' brain were processed for tyrosine hydroxylase (TH) immunohistochemical assessment.

Results: Our results showed that the chronic treatment of CAR protected against motor disabilities, reducing the time of catalepsy behavior and decreasing the frequency of oral movements, possibly by preserving TH levels in the Ventral Tegmental Area (VTA) and SNpc.

Conclusion: CAR extract is effective to attenuate motor symptoms in rats associated with increased TH+ levels in the Ventral Tegmental Area (VTA) and SNpc, indicating the potential nutraceutical benefits of CAR extract in a progressive parkinsonism model induced by RES.

紫胡萝卜提取物在利血平诱导的帕金森病模型中对黑质神经通路具有神经保护作用
背景:帕金森病(PD)是一种慢性神经退行性疾病,其特征是黑质通路中多巴胺能神经元的逐渐丧失。即使科学技术不断进步,用于治疗帕金森病的治疗方法在长期控制症状进展方面仍基本无效。开发治疗帕金森氏症的新型治疗策略的需求日益增长。不同的草药和补充剂被认为是治疗帕金森病症状的辅助药物。胡萝卜是世界上食用量最大的蔬菜品种之一,其根部因含有花青素而闻名,花青素具有抗氧化和抗炎特性。本研究评估了紫胡萝卜提取物(CAR)对雷舍平(RES)诱导的进行性帕金森病模型大鼠的神经保护作用:雄性大鼠(6 个月大)口服 CAR(400 毫克/千克)或药物,皮下注射 RES(0.01 毫克/千克)或药物 28 天(预防阶段)。从第 29 天起,大鼠每天接受 CAR 或载体治疗,隔天接受 RES(0.1 毫克/千克)或载体治疗(23 天,保护阶段)。在整个治疗过程中进行行为测试。治疗结束后,对动物大脑进行酪氨酸羟化酶(TH)免疫组化评估:结果:我们的研究结果表明,CAR的慢性治疗可防止运动障碍,减少催眠行为发生的时间,降低口腔运动的频率,这可能是通过保护被盖腹区(VTA)和SNpc中的TH水平实现的:结论:CAR提取物能有效减轻大鼠的运动症状,这些症状与大鼠腹侧被盖区(VTA)和SNpc中的TH+水平升高有关,这表明CAR提取物在RES诱导的进行性帕金森病模型中具有潜在的营养保健作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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