{"title":"Cholecalciferol Exhibits no Antibacterial Effect on <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>: An in vitro Study.","authors":"Shilia Jacob Kurian, Avirup Biswas, Shrivathsa Kulavalli, Jesil Mathew Aranjani, Amrita Pattanaik, Murali Munisamy, Kavitha Saravu, Gabriel Sunil Rodrigues, Sonal Sekhar Miraj","doi":"10.2174/0127724344277290231211051800","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The pleiotropic effect of cholecalciferol (vitamin D<sub>3</sub>) has gained significant momentum and has been explored widely.</p><p><strong>Objectives: </strong>The study aimed to investigate the antimicrobial effect of cholecalciferol against <i>S. aureus</i> and <i>E. coli</i>.</p><p><strong>Methods: </strong>An <i>in vitro</i> study was performed for the antimicrobial effect of cholecalciferol against <i>S. aureus</i> and <i>E. coli</i>. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined following the broth microdilution method.</p><p><strong>Results: </strong>The MIC value of cholecalciferol against both S. aureus and <i>E. coli</i> was 0.312 mg/ml, and the MBC for both organisms was 1.25 mg/ml. However, we also observed a significant antimicrobial effect in the dimethyl sulfoxide (DMSO) control at 12.5% (v/v). Therefore, the observed antimicrobial effect may be attributed to DMSO, indicating cholecalciferol does not directly inhibit <i>S. aureus</i> and <i>E. coli</i>.</p><p><strong>Conclusion: </strong>This study indicates that cholecalciferol does not directly inhibit S. aureus and E. coli. Hence, we suggest exploring the antibacterial properties of other vitamin D analogs, such as calcitriol or its synergetic effect with other antimicrobial agents.</p>","PeriodicalId":74643,"journal":{"name":"Recent advances in anti-infective drug discovery","volume":" ","pages":"315-321"},"PeriodicalIF":0.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Recent advances in anti-infective drug discovery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/0127724344277290231211051800","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: The pleiotropic effect of cholecalciferol (vitamin D3) has gained significant momentum and has been explored widely.
Objectives: The study aimed to investigate the antimicrobial effect of cholecalciferol against S. aureus and E. coli.
Methods: An in vitro study was performed for the antimicrobial effect of cholecalciferol against S. aureus and E. coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined following the broth microdilution method.
Results: The MIC value of cholecalciferol against both S. aureus and E. coli was 0.312 mg/ml, and the MBC for both organisms was 1.25 mg/ml. However, we also observed a significant antimicrobial effect in the dimethyl sulfoxide (DMSO) control at 12.5% (v/v). Therefore, the observed antimicrobial effect may be attributed to DMSO, indicating cholecalciferol does not directly inhibit S. aureus and E. coli.
Conclusion: This study indicates that cholecalciferol does not directly inhibit S. aureus and E. coli. Hence, we suggest exploring the antibacterial properties of other vitamin D analogs, such as calcitriol or its synergetic effect with other antimicrobial agents.
背景：胆钙化醇（维生素 D3胆钙化醇（维生素 D3）的多效作用已获得了显著的势头，并得到了广泛的探讨：本研究旨在探讨胆钙化醇对金黄色葡萄球菌和大肠杆菌的抗菌作用：方法：对胆钙化醇对金黄色葡萄球菌和大肠杆菌的抗菌效果进行了体外研究。采用肉汤微稀释法测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）：结果：胆钙醇对金黄色葡萄球菌和大肠杆菌的 MIC 值均为 0.312 mg/ml，对两种生物的 MBC 均为 1.25 mg/ml。不过，我们也观察到 12.5%（v/v）的二甲基亚砜（DMSO）对照组具有明显的抗菌效果。因此，观察到的抗菌效果可能归因于二甲基亚砜，表明胆钙化醇并不能直接抑制金黄色葡萄球菌和大肠杆菌：本研究表明，胆钙化醇并不能直接抑制金黄色葡萄球菌和大肠杆菌。因此，我们建议探索其他维生素 D 类似物的抗菌特性，如钙三醇或其与其他抗菌剂的协同作用。
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