Jin Yang , Qian Xu , Wenwen Shen , Zhe Jiang , Xinran Gu , Fanchi Li , Bing Li , Jing Wei
{"title":"The Toll/IMD pathways mediate host protection against dipteran parasitoids","authors":"Jin Yang , Qian Xu , Wenwen Shen , Zhe Jiang , Xinran Gu , Fanchi Li , Bing Li , Jing Wei","doi":"10.1016/j.jinsphys.2024.104614","DOIUrl":null,"url":null,"abstract":"<div><p>Parasitoids have utilized a variety of strategies to counteract host defense. They are in different taxonomic status and exhibit phenotypic and genetic diversity, and thus are thought to evolve distinct anti-defense mechanisms. In this study, we investigated the performance of two closely related parasitoids, <em>Exorista japonica</em> and <em>Exorista sorbillans</em> (Diptera: Tachinidae) that are biological control agents in agriculture and major insect pests in sericulture, on the host <em>Bombyx mori</em>. We show that the host is more susceptible to <em>E. sorbillans</em> infection while relatively resistant to <em>E. japonica</em> infection. Moreover, the expression levels of host antimicrobial peptides (AMPs) genes are repressed at early infection and induced at late infection of <em>E. japonica</em>, while AMPs are over-expressed at early infection and return to normal levels at late infection of <em>E. sorbillans</em>. In parallel, Toll and IMD pathway genes are generally induced at late infection of <em>E. japonica</em>, whereas these genes are up-regulated at early infection and down-regulated at late infection of <em>E. sorbillans</em>. Activating of host Toll/IMD pathways and AMPs expression by lipopolysaccharide (LPS) represses the larval growth of <em>E. sorbillans</em>. Conversely, inhibiting host Toll/IMD pathways by RNA interference significantly promotes <em>E. japonica</em> development. Therefore, the Toll/IMD pathways are required in the host for defense against infection of dipteran parasitoids. Overall, our study provides the new insight into the diversified host-parasitoid interactions, and offers a theoretical basis for further studies of the adaptive mechanism of dipteran parasitoids.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"153 ","pages":"Article 104614"},"PeriodicalIF":2.3000,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of insect physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022191024000027","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Parasitoids have utilized a variety of strategies to counteract host defense. They are in different taxonomic status and exhibit phenotypic and genetic diversity, and thus are thought to evolve distinct anti-defense mechanisms. In this study, we investigated the performance of two closely related parasitoids, Exorista japonica and Exorista sorbillans (Diptera: Tachinidae) that are biological control agents in agriculture and major insect pests in sericulture, on the host Bombyx mori. We show that the host is more susceptible to E. sorbillans infection while relatively resistant to E. japonica infection. Moreover, the expression levels of host antimicrobial peptides (AMPs) genes are repressed at early infection and induced at late infection of E. japonica, while AMPs are over-expressed at early infection and return to normal levels at late infection of E. sorbillans. In parallel, Toll and IMD pathway genes are generally induced at late infection of E. japonica, whereas these genes are up-regulated at early infection and down-regulated at late infection of E. sorbillans. Activating of host Toll/IMD pathways and AMPs expression by lipopolysaccharide (LPS) represses the larval growth of E. sorbillans. Conversely, inhibiting host Toll/IMD pathways by RNA interference significantly promotes E. japonica development. Therefore, the Toll/IMD pathways are required in the host for defense against infection of dipteran parasitoids. Overall, our study provides the new insight into the diversified host-parasitoid interactions, and offers a theoretical basis for further studies of the adaptive mechanism of dipteran parasitoids.
期刊介绍:
All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.