{"title":"Bacterial communities in the potato tuberosphere share similarities with bulk soil and rhizosphere communities, yet possess distinct features","authors":"Georgia Voulgari, Achim Schmalenberger","doi":"10.1016/j.pedobi.2024.150935","DOIUrl":null,"url":null,"abstract":"<div><p>To date, a paucity of studies have examined bacterial communities in tuberospheres. However, the function of these bacterial communities in healthy potato plants is still largely unknown. Here, we aimed to describe the structure and composition of tuberosphere bacteriomes and its capability to make organosulfur plant available using the rhizosphere and bulk soil bacterial community characteristics as a reference. Rhizosphere, tuberosphere and bulk soil was collected from two field grown potato varieties. Bacterial communities were characterized by 16S rRNA gene amplicon sequencing. Bacterial organosulfur mobilization indicators were evaluated with cultivation dependent and independent methods and were correlated (Spearman) with the relative abundance of bacterial families. The structure of tuberosphere bacterial communities either overlapped with the bulk soil or had similarities with the rhizosphere. Relative abundance of specific bacterial families were distinct between bulk soil, tuberosphere and rhizosphere. Tuberospheres had a tendency for higher arylsulfonate utilization compared to bulk soil. The families <em>Sphingomonadaceae, Sphingobacteriaceae</em> and <em>Rhizobiaceae</em> which presented a decline in their relative abundances from the rhizosphere to tuberosphere and bulk soil had positive correlations with organosulfur mobilizing indicators. Potato variety and soil characteristics played a role in structuring the tuberosphere bacterial communities. Tuberospheres represent an environment in-between bulk soil and rhizosphere indicative from the intermediate relative abundances of specific bacterial families. A moderately higher bacterial organosulfur mobilization activity in tuberospheres suggests that this microbial function may serve specific biological roles for potato tubers.</p></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0031405624009065/pdfft?md5=f58313b2f1df9faee4bf76c177eb9d52&pid=1-s2.0-S0031405624009065-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pedobiologia","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0031405624009065","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
To date, a paucity of studies have examined bacterial communities in tuberospheres. However, the function of these bacterial communities in healthy potato plants is still largely unknown. Here, we aimed to describe the structure and composition of tuberosphere bacteriomes and its capability to make organosulfur plant available using the rhizosphere and bulk soil bacterial community characteristics as a reference. Rhizosphere, tuberosphere and bulk soil was collected from two field grown potato varieties. Bacterial communities were characterized by 16S rRNA gene amplicon sequencing. Bacterial organosulfur mobilization indicators were evaluated with cultivation dependent and independent methods and were correlated (Spearman) with the relative abundance of bacterial families. The structure of tuberosphere bacterial communities either overlapped with the bulk soil or had similarities with the rhizosphere. Relative abundance of specific bacterial families were distinct between bulk soil, tuberosphere and rhizosphere. Tuberospheres had a tendency for higher arylsulfonate utilization compared to bulk soil. The families Sphingomonadaceae, Sphingobacteriaceae and Rhizobiaceae which presented a decline in their relative abundances from the rhizosphere to tuberosphere and bulk soil had positive correlations with organosulfur mobilizing indicators. Potato variety and soil characteristics played a role in structuring the tuberosphere bacterial communities. Tuberospheres represent an environment in-between bulk soil and rhizosphere indicative from the intermediate relative abundances of specific bacterial families. A moderately higher bacterial organosulfur mobilization activity in tuberospheres suggests that this microbial function may serve specific biological roles for potato tubers.
迄今为止,对块茎球中细菌群落的研究还很少。然而,这些细菌群落在健康马铃薯植株中的功能在很大程度上仍然未知。在此,我们旨在以根瘤菌层和大块土壤细菌群落特征为参考,描述块茎层细菌群落的结构和组成及其使植物获得有机硫的能力。通过 16 S rRNA 基因扩增片段测序确定了细菌群落的特征。细菌有机硫动员指标用依赖于栽培和独立于栽培的方法进行评估,并与细菌家族的相对丰度相关(Spearman)。块茎圈细菌群落的结构要么与块茎土壤重叠,要么与根瘤菌圈相似。块茎土壤、块茎圈和根瘤菌圈中特定细菌家族的相对丰度各不相同。与大块土壤相比,块茎圈对芳基磺酸盐的利用率更高。鞘氨单胞菌科(Sphingomonadaceae)、鞘氨杆菌科(Sphingobacteriaceae)和根瘤菌科(Rhizobiaceae)的相对丰度从根瘤层到块茎层和大块土壤均呈下降趋势,它们与有机硫动员指标呈正相关。块茎层代表了介于大块土壤和根瘤层之间的环境,表明了特定细菌家族的中间相对丰度。块茎圈中的细菌有机硫动员活性略高,这表明这种微生物功能可能对马铃薯块茎具有特殊的生物学作用。
期刊介绍:
Pedobiologia publishes peer reviewed articles describing original work in the field of soil ecology, which includes the study of soil organisms and their interactions with factors in their biotic and abiotic environments.
Analysis of biological structures, interactions, functions, and processes in soil is fundamental for understanding the dynamical nature of terrestrial ecosystems, a prerequisite for appropriate soil management. The scope of this journal consists of fundamental and applied aspects of soil ecology; key focal points include interactions among organisms in soil, organismal controls on soil processes, causes and consequences of soil biodiversity, and aboveground-belowground interactions.
We publish:
original research that tests clearly defined hypotheses addressing topics of current interest in soil ecology (including studies demonstrating nonsignificant effects);
descriptions of novel methodological approaches, or evaluations of current approaches, that address a clear need in soil ecology research;
innovative syntheses of the soil ecology literature, including metaanalyses, topical in depth reviews and short opinion/perspective pieces, and descriptions of original conceptual frameworks; and
short notes reporting novel observations of ecological significance.