Vancomycin-variable enterococci in sheep and cattle isolates and whole-genome sequencing analysis of isolates harboring vanM and vanB genes.

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2023-01-01 DOI:10.22099/IJVR.2023.47465.6855

B Onaran Acar, G Cengız, M Goncuoglu

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引用次数: 0

Abstract

Background: Vancomycin resistance encoded by the vanA/B/M genes in enterococci is clinically important because of the transmission of these genes between bacteria. While vancomycin resistance is determined by detecting only vanA and vanB genes by routine analyses, failure to detect vanM resistance causes vancomycin resistance to be overlooked, and clinically appropriate treatment cannot be provided.

Aims: The study aimed to examine the presence of vanM-positive enterococcal isolates in Ankara, Turkey, and to have detailed information about them with sequence analyses.

Methods: Caecal samples were collected from sheep and cattle during slaughter at different slaughterhouses in Ankara, Turkey. Enterococci isolates were identified, confirmed, and analyzed for the presence of vanA/B/M genes. Antibiotic resistance profiles of isolates were determined by the broth microdilution method. A whole genome sequence analysis of the isolates harboring the vanM and vanB genes was performed.

Results: 13.7% of enterococcal isolates were determined as Enterococcus faecium and Enterococcus faecalis. 15% of these isolates contained vanB, and 40% were vanM-positive. S98b and C32 isolates were determined to contain 16 CRISPR-Cas elements. 80% of the enterococci isolates were resistant to nitrofurantoin and 15% to ciprofloxacin. The first vanM-positive vancomycin-variable enterococci (VVE) isolates from food-producing animals were identified, and the S98b strain has been assigned to Genbank with the accession number CP104083.1.

Conclusion: Therefore, new studies are needed to facilitate the identification of vanM-resistant enterococci and VVE strains.

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羊和牛分离物中的万古霉素变异肠球菌以及对携带 vanM 和 vanB 基因的分离物进行全基因组测序分析。

背景：肠球菌中由 vanA/B/M 基因编码的万古霉素耐药性在临床上非常重要，因为这些基因会在细菌之间传播。常规分析仅通过检测 vanA 和 vanB 基因来确定万古霉素耐药性，但如果不能检测出 vanM 耐药性，就会导致万古霉素耐药性被忽视，从而无法提供临床上适当的治疗。研究目的：本研究旨在检测土耳其安卡拉地区是否存在 vanM 阳性的肠球菌分离物，并通过序列分析获得有关这些分离物的详细信息：方法：在土耳其安卡拉不同屠宰场屠宰绵羊和牛时采集其粪便样本。对分离出的肠球菌进行鉴定、确认并分析其是否含有 vanA/B/M 基因。采用肉汤微稀释法测定分离物的抗生素耐药性。对携带 vanM 和 vanB 基因的分离株进行了全基因组序列分析：结果：13.7% 的肠球菌分离物被确定为粪肠球菌和粪肠球菌。这些分离物中有 15% 含有 vanB 基因，40% 为 vanM 阳性。S98b 和 C32 分离物被确定含有 16 个 CRISPR-Cas 元件。80%的肠球菌分离物对硝基呋喃妥因耐药，15%对环丙沙星耐药。首次从食用动物中鉴定出了vanM阳性的万古霉素变异性肠球菌（VVE）分离株，S98b菌株已被归入Genbank，登录号为CP104083.1：因此，需要开展新的研究，以促进耐 vanM 肠球菌和 VVE 菌株的鉴定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.