Extracellular vesicles from Lacticaseibacillus paracasei PC-H1 inhibit HIF-1α-mediated glycolysis of colon cancer.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2024-02-01 Epub Date: 2024-01-25 DOI:10.2217/fmb-2023-0144

Yangqian Shi, Chunliang Zhang, Wanyu Cao, Luyi Li, Kaili Liu, Hanyue Zhu, Fikadu Balcha, Yong Fang

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引用次数: 0

Abstract

Aims: Extracellular vesicles from Lacticaseibacillus paracasei PC-H1 have antiproliferative activity of colon cells, but the effect on glycolytic metabolism of cancer cell remains enigmatic. The authors investigated how Lacticaseibacillus paracasei extracellular vesicles (LpEVs) inhibit the growth of colon cancer cells by affecting tumor metabolism. Materials & methods: HCT116 cells were treated with LpEVs and then differentially expressed genes were analyzed by transcriptome sequencing, the sequencing results were confirmed in vivo and in vitro. Results: LpEVs entered colon cancer cells and inhibited their growth. Transcriptome sequencing revealed differentially expressed genes were related to glycolysis. Lactate production, glucose uptake and lactate dehydrogenase activity were significantly reduced after treatment. LpEVs also reduced HIF-1α, GLUT1 and LDHA expression. Conclusion: LpEVs exert their antiproliferative activity of colon cancer cells by decreasing HIF-1α-mediated glycolysis.

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副酸乳杆菌 PC-H1 的细胞外囊泡抑制 HIF-1α 介导的结肠癌糖酵解。

目的：副酸乳杆菌（Lacticaseibacillus paracasei）PC-H1的胞外囊泡具有抗结肠细胞增殖的活性，但其对癌细胞糖代谢的影响仍然是个谜。作者研究了副酸乳杆菌胞外囊泡（LpEVs）如何通过影响肿瘤代谢来抑制结肠癌细胞的生长。材料与方法：用 LpEVs 处理 HCT116 细胞，然后通过转录组测序分析差异表达基因，测序结果在体内和体外均得到证实。结果LpEVs进入结肠癌细胞并抑制其生长。转录组测序显示，差异表达的基因与糖酵解有关。处理后，乳酸生成、葡萄糖摄取和乳酸脱氢酶活性显著降低。LpEVs 还降低了 HIF-1α、GLUT1 和 LDHA 的表达。结论LpEVs 通过降低 HIF-1α 介导的糖酵解，发挥其抗结肠癌细胞增殖的活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.