Oral arsenite exposure induces inflammation and apoptosis in pulmonary tissue: acute and chronic evaluation in young and adult mice

IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Samata Pradhan, Syed Afroz Ali, Mahesh Rachamalla, Som Niyogi, Ashok Kumar Datusalia
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Abstract

Inorganic arsenic is a well-known environmental toxicant, and exposure to this metalloid is strongly linked with severe and extensive toxic effects in various organs including the lungs. In the present study, we aimed to investigate the acute and chronic effects of arsenite exposure on pulmonary tissue in young and adult mice. In brief, young and adult female Balb/C mice were exposed to 3 and 30 ppm arsenite daily via drinking water for 30 and 90 days. Subsequently, the animals were sacrificed and various histological and immunohistochemistry (IHC) analyses were performed using lung tissues. Our findings showed arsenite was found to cause dose-dependent pathological changes such as thickening of the alveolar septum, inflammatory cell infiltrations and lung fibrosis in young and adult mice. In addition, arsenite exposure significantly increased the expression of inflammatory markers NF-κB and TNF-α, indicating that arsenite-exposed mice suffered from severe lung inflammation. Moreover, the IHC analysis of fibrotic proteins demonstrated an increased expression of TGF-β1, α-SMA, vimentin and collagen-I in the arsenite-exposed mice compared to the control mice. This was accompanied by apoptosis, which was indicated by the upregulated expression of caspase-3 in arsenite-exposed mice compared to the control. Adult mice were generally found to be more prone to arsenite toxicity during chronic exposure relative to their younger counterparts. Overall, our findings suggest that arsenite in drinking water may induce dose-dependent and age-dependent structural and functional impairment in the lungs through elevating inflammation and fibrotic proteins.

Abstract Image

口服亚砷酸盐会诱发肺组织炎症和细胞凋亡:对幼年和成年小鼠进行急性和慢性评估。
无机砷是一种众所周知的环境毒物,接触这种类金属会对包括肺部在内的多个器官产生严重而广泛的毒性影响。本研究旨在探讨亚砷酸盐暴露对幼年和成年小鼠肺组织的急性和慢性影响。简而言之,每天通过饮用水将幼年和成年雌性 Balb/C 小鼠暴露于 3 和 30 ppm 的亚砷酸盐中,分别持续 30 天和 90 天。随后,动物被处死,并利用肺组织进行各种组织学和免疫组化(IHC)分析。我们的研究结果表明,亚砷酸盐会导致幼年和成年小鼠肺泡间隔增厚、炎症细胞浸润和肺纤维化等剂量依赖性病理变化。此外,亚砷酸盐暴露会明显增加炎症标志物 NF-κB 和 TNF-α 的表达,表明亚砷酸盐暴露的小鼠患有严重的肺部炎症。此外,对纤维化蛋白的 IHC 分析表明,与对照组小鼠相比,暴露于亚砷酸盐的小鼠中 TGF-β1、α-SMA、波形蛋白和胶原蛋白-I 的表达增加。与对照组相比,暴露于亚砷酸盐的小鼠体内 Caspase-3 的表达上调,表明伴随着细胞凋亡。在长期接触亚砷酸盐的过程中,成年小鼠通常比年轻小鼠更容易受到亚砷酸盐的毒性影响。总之,我们的研究结果表明,饮用水中的亚砷酸盐可能会通过升高炎症和纤维化蛋白,诱发剂量依赖性和年龄依赖性的肺部结构和功能损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biometals
Biometals 生物-生化与分子生物学
CiteScore
5.90
自引率
8.60%
发文量
111
审稿时长
3 months
期刊介绍: BioMetals is the only established journal to feature the important role of metal ions in chemistry, biology, biochemistry, environmental science, and medicine. BioMetals is an international, multidisciplinary journal singularly devoted to the rapid publication of the fundamental advances of both basic and applied research in this field. BioMetals offers a forum for innovative research and clinical results on the structure and function of: - metal ions - metal chelates, - siderophores, - metal-containing proteins - biominerals in all biosystems. - BioMetals rapidly publishes original articles and reviews. BioMetals is a journal for metals researchers who practice in medicine, biochemistry, pharmacology, toxicology, microbiology, cell biology, chemistry, and plant physiology who are based academic, industrial and government laboratories.
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