Characterization of the complete mitochondrial genome and phylogenetic analyses of Haemaphysalis tibetensis Hoogstraal, 1965 (Acari: Ixodidae)

IF 3.1 2区医学 Q2 INFECTIOUS DISEASES

Ticks and Tick-borne Diseases Pub Date : 2024-01-22 DOI:10.1016/j.ttbdis.2024.102311

Wenqiang Tang , Xin Li , Bijin Ye , Bin Shi , Haoji Zhang , Zhisheng Dang , Yuexiang Sun , Lamu Danqu , Chenyang Xia , Danzeng Quzhen , Xialing Zhao , Wenting Chui , Fuqiang Huang

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引用次数: 0

Abstract

Ticks are specialized ectoparasites that feed on blood, causing physical harm to the host and facilitating pathogen transmission. The genus Haemaphysalis contains vectors for numerous infectious agents. These agents cause various diseases in humans and animals. Mitochondrial genome sequences serve as reliable molecular markers, forming a crucial basis for evolutionary analyses, studying species origins, and exploring molecular phylogeny. We extracted mitochondrial genome from the enriched mitochondria of Haemaphysalis tibetensis and obtained a 14,714-bp sequence. The mitochondrial genome consists of 13 protein-coding genes (PCGs), two ribosomal RNA, 22 transfer RNAs (tRNAs), and two control regions. The nucleotide composition of H. tibetensis mitochondrial genome was 38.38 % for A, 9.61 % for G, 39.32 % for T, and 12.69 % for C. The A + T content of H. tibetensis mitochondrial genome was 77.7 %, significantly higher than the G + C content. The repeat units of H. tibetensis exhibited two identical repeat units of 33 bp in length, positioned downstream of nad1 and rrnL genes. Furthermore, phylogenetic analyses based on the 13 PCGs indicated that Haemaphysalis tibetensis (subgenus Allophysalis) formed a monophyletic clade with Haemaphysalis nepalensis (subgenus Herpetobia) and Haemaphysalis danieli (subgenus Allophysalis). Although the species Haemaphysalis inermis, Haemaphysalis kitaokai, Haemaphysalis kolonini, and Haemaphysalis colasbelcouri belong to the subgenus Alloceraea, which were morphologically primitive hemaphysalines just like H. tibetensis, these four tick species cannot form a single clade with H. tibetensis. In this study, the whole mitochondrial genome sequence of H. tibetensis from Tibet was obtained, which enriched the mitochondrial genome data of ticks and provided genetic markers to study the population heredity and molecular evolution of the genus Haemaphysalis.

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Haemaphysalis tibetensis Hoogstraal, 1965（Acari: Ixodidae）完整线粒体基因组的特征和系统发育分析。

蜱虫是专门的体外寄生虫，以血液为食，对宿主造成身体伤害，并促进病原体传播。蜱属（Haemaphysalis）包含多种传染性病原体的载体。这些病原体会导致人类和动物患上各种疾病。线粒体基因组序列是可靠的分子标记，是进行进化分析、研究物种起源和探索分子系统发育的重要依据。我们从富集的西藏毛腿虫线粒体中提取了线粒体基因组，获得了14714bp的序列。线粒体基因组由13个蛋白质编码基因（PCGs）、2个核糖体RNA、22个转运RNA（tRNAs）和2个控制区组成。线粒体基因组的核苷酸组成为：A 占 38.38%，G 占 9.61%，T 占 39.32%，C 占 12.69%；线粒体基因组中 A + T 的含量为 77.7%，明显高于 G + C 的含量。H. tibetensis 的重复单元有两个长度为 33 bp 的相同重复单元，位于 nad1 和 rrnL 基因的下游。此外，基于 13 个 PCGs 的系统进化分析表明，西藏毛蛛（Allophysalis 亚属）与尼泊尔毛蛛（Haemaphysalis nepalensis 亚属）和丹尼尔毛蛛（Haemaphysalis danieli 亚属）形成了一个单系支系。虽然Haemaphysalis inermis、Haemaphysalis kitaokai、Haemaphysalis kolonini和Haemaphysalis colasbelcouri属于Alloceraea亚属，在形态上与西藏蜱一样是原始的半蜱，但这4种蜱不能与西藏蜱组成一个支系。本研究获得了西藏蜱线粒体全基因组序列，丰富了蜱线粒体基因组数据，为研究西藏蜱属的种群遗传和分子进化提供了遗传标记。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ticks and Tick-borne Diseases INFECTIOUS DISEASES-MICROBIOLOGY

CiteScore

6.90

自引率

12.50%

发文量

185

审稿时长

6-12 weeks

期刊介绍： Ticks and Tick-borne Diseases is an international, peer-reviewed scientific journal. It publishes original research papers, short communications, state-of-the-art mini-reviews, letters to the editor, clinical-case studies, announcements of pertinent international meetings, and editorials. The journal covers a broad spectrum and brings together various disciplines, for example, zoology, microbiology, molecular biology, genetics, mathematical modelling, veterinary and human medicine. Multidisciplinary approaches and the use of conventional and novel methods/methodologies (in the field and in the laboratory) are crucial for deeper understanding of the natural processes and human behaviour/activities that result in human or animal diseases and in economic effects of ticks and tick-borne pathogens. Such understanding is essential for management of tick populations and tick-borne diseases in an effective and environmentally acceptable manner.