Development and characterization of potential larvicidal nanoemulsions against Aedes aegypti.

IF 2.6 4区 材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY
Beilstein Journal of Nanotechnology Pub Date : 2024-01-18 eCollection Date: 2024-01-01 DOI:10.3762/bjnano.15.10
Jonatas L Duarte, Leonardo Delello Di Filippo, Anna Eliza Maciel de Faria Mota Oliveira, Rafael Miguel Sábio, Gabriel Davi Marena, Tais Maria Bauab, Cristiane Duque, Vincent Corbel, Marlus Chorilli
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引用次数: 0

Abstract

Plant-based insecticides offer advantages such as negligible residual effects, reduced risks to both humans and the environment, and immunity to resistance issues that plague conventional chemicals. However, the practical use of monoterpenes in insect control has been hampered by challenges including their poor solubility and stability in aqueous environments. In recent years, the application of nanotechnology-based formulations, specifically nanoemulsions, has emerged as a prospective strategy to surmount these obstacles. In this study, we developed and characterized nanoemulsions based on cymene and myrcene and assessed their toxicity both in vitro using human keratinocytes (HaCAT) cells and in an in vivo model involving Galleria mellonella larvae. Additionally, we investigated the insecticidal efficacy of monoterpenes against the mosquito Aedes aegypti, the primary dengue vector, via larval bioassay. Employing a low-energy approach, we successfully generated nanoemulsions. The cymene-based nanoemulsion exhibited a hydrodynamic diameter of approximately 98 nm and a zeta potential of -25 mV. The myrcene-based nanoemulsion displayed a hydrodynamic diameter of 118 nm and a zeta potential of -20 mV. Notably, both nanoemulsions demonstrated stability over 60 days, accompanied by controlled release properties and low toxicity towards HaCAT cells and Galleria mellonella larvae. Moreover, the nanoemulsions exhibited significant lethality against third-instar Aedes aegypti larvae at a concentration of 50 mg/L. In conclusion, the utilization of nanoemulsions encapsulating cymene and myrcene presents a promising avenue for overcoming the limitations associated with poor solubility and stability of monoterpenes. This study sheds light on the potential of the nanoemulsions as effective and environmentally friendly insecticides in the ongoing battle against mosquito-borne diseases.

针对埃及伊蚊的潜在杀幼虫剂纳米乳剂的开发和特性分析。
以植物为基础的杀虫剂具有多种优势,例如残留影响微乎其微、对人类和环境的风险较低、不受困扰传统化学品的抗药性问题的影响。然而,单萜烯类化合物在水环境中的溶解性和稳定性较差等难题阻碍了其在昆虫控制领域的实际应用。近年来,应用基于纳米技术的制剂,特别是纳米乳剂,已成为克服这些障碍的一种前瞻性策略。在本研究中,我们开发并鉴定了基于苏木烯和月桂烯的纳米乳液,并利用人体角质细胞(HaCAT)进行了体外毒性评估,同时还采用了一种涉及灰飞虱幼虫的体内模型。此外,我们还通过幼虫生物测定研究了单萜烯类化合物对埃及伊蚊(登革热的主要传播媒介)的杀虫功效。采用低能耗方法,我们成功地生成了纳米乳剂。基于亚麻仁的纳米乳液的流体力学直径约为 98 nm,zeta 电位为 -25 mV。亚麻仁基纳米乳液的流体力学直径为 118 nm,zeta 电位为 -20 mV。值得注意的是,这两种纳米乳液在 60 天内都表现出稳定性,同时具有控释特性,对 HaCAT 细胞和 Galleria mellonella 幼虫的毒性较低。此外,在 50 毫克/升的浓度下,纳米乳剂对埃及伊蚊三龄幼虫有显著的致死作用。总之,利用包裹环烯和月桂烯的纳米乳剂是克服单萜烯类化合物溶解性和稳定性差所带来的局限性的一条很有前景的途径。这项研究揭示了纳米乳剂作为有效、环保的杀虫剂在当前防治蚊媒疾病的斗争中的潜力。
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来源期刊
Beilstein Journal of Nanotechnology
Beilstein Journal of Nanotechnology NANOSCIENCE & NANOTECHNOLOGY-MATERIALS SCIENCE, MULTIDISCIPLINARY
CiteScore
5.70
自引率
3.20%
发文量
109
审稿时长
2 months
期刊介绍: The Beilstein Journal of Nanotechnology is an international, peer-reviewed, Open Access journal. It provides a unique platform for rapid publication without any charges (free for author and reader) – Platinum Open Access. The content is freely accessible 365 days a year to any user worldwide. Articles are available online immediately upon publication and are publicly archived in all major repositories. In addition, it provides a platform for publishing thematic issues (theme-based collections of articles) on topical issues in nanoscience and nanotechnology. The journal is published and completely funded by the Beilstein-Institut, a non-profit foundation located in Frankfurt am Main, Germany. The editor-in-chief is Professor Thomas Schimmel – Karlsruhe Institute of Technology. He is supported by more than 20 associate editors who are responsible for a particular subject area within the scope of the journal.
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