M. Bochniarz, Agata Hahaj-Siembida, M. Krajewska-Wędzina, Marcelina Osińska, Anna Tracz, A. Trościańczyk, P. Brodzki, L. Krakowski, U. Kosior-Korzecka, A. Nowakiewicz
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引用次数: 0
Abstract
The aim of the study was evaluation of the concentrations of interleukin (IL)-1β, IL-8, IL-12β and tumour necrosis factor alpha (TNF-α) in the serum and milk of cows with mastitis caused by Streptococcus agalactiae.
A total of 60 milk samples from diseased cows and 30 milk samples from healthy cows were included in the study. Blood and milk samples were taken from Holstein-Friesian cows from three herds (two in tie-stall and one in a free-stall housing system) in Lublin Province in Poland. The concentrations of cytokines in blood serum and quarter milk samples were determined by ELISA.
The levels of IL-1β, IL-8, IL-12β and TNF-α were significantly higher in the milk of cows suffering from mastitis caused by S. agalactiae compared to the milk of healthy cows (263.03 vs 55.36 pg/mL, 298.34 vs 131.82 pg/mL, 604.10 vs 139.17 pg/mL and 460.86 vs 78.82 pg/mL, respectively). In the group of sick cows, cytokine levels were significantly higher in milk than in serum (263.03 vs 55.25 pg/mL for IL-1β, 298.34 vs 164.22 pg/mL for IL-8, 604.10 vs 70.34 pg/mL for IL-12β and 460.86 vs 104.78 pg/mL for TNF-α).
The results confirm the involvement of the entire bovine immune system to protect against the bacteria first locally in the udder. The response of the mammary gland to infection caused by S. agalactiae is rapid and already very strong at the beginning of the infection.
该研究的目的是评估白细胞介素(IL)-1β、IL-8、IL-12β和肿瘤坏死因子α(TNF-α)在由无乳链球菌引起的乳腺炎奶牛血清和牛奶中的浓度。 研究共纳入了 60 份患病奶牛的牛奶样本和 30 份健康奶牛的牛奶样本。血液和牛奶样本取自波兰卢布林省三个牧场的荷斯坦-弗里斯兰奶牛(其中两个牧场采用扎栏饲养,一个牧场采用散栏饲养)。血清和四分之一牛奶样本中的细胞因子浓度是通过 ELISA 法测定的。 与健康奶牛的乳汁相比,由S. agalactiae引起的乳腺炎奶牛的乳汁中IL-1β、IL-8、IL-12β和TNF-α的水平明显更高(分别为263.03 vs 55.36 pg/mL、298.34 vs 131.82 pg/mL、604.10 vs 139.17 pg/mL和460.86 vs 78.82 pg/mL)。在病牛组,牛奶中的细胞因子水平明显高于血清(IL-1β为263.03 vs 55.25 pg/mL,IL-8为298.34 vs 164.22 pg/mL,IL-12β为604.10 vs 70.34 pg/mL,TNF-α为460.86 vs 104.78 pg/mL)。 这些结果证实,牛的整个免疫系统都参与了保护乳房免受细菌的侵害。乳腺对由S. agalactiae引起的感染的反应很快,而且在感染初期就已经非常强烈。
期刊介绍:
Journal of Veterinary Research (formerly Bulletin of the Veterinary Institute in Pulawy) is a quarterly that publishes original papers, review articles and short communications on bacteriology, virology, parasitology, immunology, molecular biology, pathology, toxicology, pharmacology, and biochemistry. The main emphasis is, however, on infectious diseases of animals, food safety and public health, and clinical sciences.