EFFECT OF TOTAL PHENOLIC AND TOTAL FLAVONOID LEVELS ON THE ANTIOXIDANT POWER OF WATER EXTRACT, ETHANOL AND CHLOROFORM OF GREEN TEA LEAVES (Camellia sinensis L)

Medical Sains : Jurnal Ilmiah Kefarmasian Pub Date : 2024-01-20 DOI:10.37874/ms.v9i1.940

Ahwan Ahwan, Anik Suwarni, Reni Ariastuti, Rosyadah Hafidz, Sinta Mei Enjelina

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Abstract

Tea (Camellia sinensis L.) was originally used as a refresh drink. Tea has many benefits, especially for health and beauty. The most widely used tea in the market is green tea, because its properties can be used as a daily drink to improve the quality of life in carrying out daily activities. The aim of this study was to determine the effect of the relationship between total phenolic and total flavonoid levels in water extract (EA), ethanol (EE), and chlorophorom (EK) of green tea leaves on antioxidant power. This experimental study used the extraction method, qualitative analysis, quantitative analysis using the colorimetric method, and antioxidant power using the DPPH method. The data obtained were statistically analyzed using SPPS and Microsoft Excel. The results obtained in The extraction process yielded extracts: EA (21.44%), EE (11.20 %), and EK (3.58 %). Qualitative analysis of flavonoid and phenolic compounds in all the extracts revealed the presence of these compounds. In the total phenolic test, the levels were: 229.07 ± 0.99 (EA); 573.70 ± 3.93 (EE); 45.27 ± 0.73 mg/g (EK) GAE (Galic Acid Equivalent). Meanwhile, the flavonoid content was 13.10 ± 0.31 (EA); 40.49 ± 0.67 (EE); 20.94 ± 0.50 mg/g (EK) QE (Quercetin Equivalent). The antioxidant results have an IC50 (Inhibitory Concentration) value of 34.64 ± 0.74 (EA); 7.02 ± 0.15 (EE); 99.11 ± 1.23; positive control 5.94 ± 0.07 µg/mL (vitamin C) ...

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总酚和总芳香烃含量对绿茶叶（Camellia sinensis L）水提取物、乙醇和氯仿抗氧化能力的影响

茶（Camellia sinensis L.）最初被用作提神饮料。茶有很多好处，特别是对健康和美容。市场上使用最广泛的茶叶是绿茶，因为它的特性可以作为日常饮品，在进行日常活动时提高生活质量。本研究旨在确定绿茶叶水提取物（EA）、乙醇（EE）和氯酚（EK）中总酚和总黄酮含量之间的关系对抗氧化能力的影响。本实验研究采用提取法、定性分析法、比色法定量分析法和 DPPH 法进行抗氧化能力分析。获得的数据使用 SPPS 和 Microsoft Excel 进行了统计分析。提取过程中得到的提取物结果如下EA（21.44%）、EE（11.20%）和 EK（3.58%）。对所有提取物中的类黄酮和酚类化合物进行的定性分析显示，这些化合物都存在。在总酚测试中，其含量为229.07 ± 0.99（EA）；573.70 ± 3.93（EE）；45.27 ± 0.73 mg/g（EK）GAE（伽利酸当量）。同时，黄酮含量为 13.10 ± 0.31（EA）；40.49 ± 0.67（EE）；20.94 ± 0.50 mg/g（EK）QE（槲皮素当量）。抗氧化结果的 IC50（抑制浓度）值为 34.64 ± 0.74（EA）；7.02 ± 0.15（EE）；99.11 ± 1.23；阳性对照为 5.94 ± 0.07 µg/mL （维生素 C）。

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Medical Sains : Jurnal Ilmiah Kefarmasian

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