Spider silk enhanced tissue engineering of cartilage tissue: Approach of a novel bioreactor model using adipose derived stromal cells.

IF 3.1 4区 医学 Q2 BIOPHYSICS
Sarah Strauß, Maximilian Diemer, Vesna Bucan, Jörn W Kuhbier, Tomke Asendorf, Peter M Vogt, Frederik Schlottmann
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引用次数: 0

Abstract

Human cartilage tissue remains a challenge for the development of therapeutic options due to its poor vascularization and reduced regenerative capacities. There are a variety of research approaches dealing with cartilage tissue engineering. In addition to different biomaterials, numerous cell populations have been investigated in bioreactor-supported experimental setups to improve cartilage tissue engineering. The concept of the present study was to investigate spider silk cocoons as scaffold seeded with adipose-derived stromal cells (ASC) in a custom-made bioreactor model using cyclic axial compression to engineer cartilage-like tissue. For chemical induction of differentiation, BMP-7 and TGF-β2 were added and changes in cell morphology and de-novo tissue formation were investigated using histological staining to verify chondrogenic differentiation. By seeding spider silk cocoons with ASC, a high colonization density and cell proliferation could be achieved. Mechanical induction of differentiation using a newly established bioreactor model led to a more roundish cell phenotype and new extracellular matrix formation, indicating a chondrogenic differentiation. The addition of BMP-7 and TGF-β2 enhanced the expression of cartilage specific markers in immunohistochemical staining. Overall, the present study can be seen as pilot study and valuable complementation to the published literature.

蜘蛛丝增强软骨组织工程:使用脂肪基质细胞的新型生物反应器模型的方法。
人体软骨组织的血管化程度低,再生能力差,因此在开发治疗方案方面仍面临挑战。软骨组织工程学的研究方法多种多样。除了不同的生物材料,人们还在生物反应器支持的实验装置中研究了多种细胞群,以改善软骨组织工程。本研究的概念是研究在一个定制的生物反应器模型中,用蛛丝茧作为支架,播种脂肪基质细胞(ASC),利用循环轴向压缩来设计软骨样组织。为了进行化学诱导分化,加入了 BMP-7 和 TGF-β2,并使用组织学染色法研究了细胞形态和新生组织形成的变化,以验证软骨分化。通过向蛛丝茧中播种 ASC,可以获得较高的定植密度和细胞增殖。利用新建立的生物反应器模型进行机械诱导分化,可使细胞表型更加圆润,并形成新的细胞外基质,这表明了软骨分化。BMP-7 和 TGF-β2 的添加增强了免疫组化染色中软骨特异性标志物的表达。总之,本研究可视为一项试验性研究,是对已发表文献的宝贵补充。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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