Comparative effects of the antioxidant glutathione with metformin and Diane-35 on hormonal, metabolic, and inflammatory indicators in a DHEA-induced PCOS rat model.

IF 2 4区 医学 Q3 ENDOCRINOLOGY & METABOLISM
Gynecological Endocrinology Pub Date : 2024-12-01 Epub Date: 2024-01-22 DOI:10.1080/09513590.2024.2302086
Aslı Coşar, Pınar Özcan, Fatma Basak Tanoglu, Olgu Enis Tok, Gülçin Özkara, Hikmet Tunç Timur, Çağlar Çetin, Duygu Neccar
{"title":"Comparative effects of the antioxidant glutathione with metformin and Diane-35 on hormonal, metabolic, and inflammatory indicators in a DHEA-induced PCOS rat model.","authors":"Aslı Coşar, Pınar Özcan, Fatma Basak Tanoglu, Olgu Enis Tok, Gülçin Özkara, Hikmet Tunç Timur, Çağlar Çetin, Duygu Neccar","doi":"10.1080/09513590.2024.2302086","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Comparison of hormonal, metabolic and inflammatory markers of glutathione with metformin and Diane-35 in a rat model of PCOS induced by dehydroepiandrosterone.</p><p><strong>Methods: </strong>Twenty-five female rats were randomized into four groups. Group 1 was administered a subcutaneous dose of 0.2 ml saline/day. Group 2 was given 0.2 ml of 1% carboxymethyl cellulose (CMC)/day orally for 28 days. A PCOS model was established with DHEA in rats. Group 3 was given 4.5 mg/kg/day of Diane-35 orally dissolved in 1% CMC for 28 days. Group 4 was given 300 mg/kg/day of metformin orally dissolved in 1 ml of saline for 28 days, and Group 5 was administered 100 mg/kg of glutathione intraperitoneally on days 35, 42, and 49. On day 56, the rats were sacrificed. Serum markers and follicle count were examined.</p><p><strong>Results: </strong>Serum IL-6, hs-CRP, insulin, testosterone, SHBG, and MDA values were significantly lower in the glutathione group than in the PCOS group (<i>p</i> = 0.0006, <i>p</i> = 0.023, <i>p</i> = 0.0082, <i>p</i> = 0.0007, <i>p</i> = 0.0048, and <i>p</i> < 0.0001, respectively).The number of all follicles was similar between the control and glutathione groups (<i>p</i> < 0.05). When we compared the other groups with the PCOS group, the number of primary, secondary, atretic, and cystic follicles was significantly lower in the metformin and glutathione groups. The number of primordial and antral follicles was significantly higher than in the PCOS group.</p><p><strong>Conclusions: </strong>Glutathione plays anti-inflammatory and antioxidant roles, similar to metformin, by lowering serum IL-6, insulin, testosterone, CRP, and MDA levels; decreasing atretic/cystic follicle count; and improving antral follicle count and folliculogenesis in PCOS patients.</p>","PeriodicalId":12865,"journal":{"name":"Gynecological Endocrinology","volume":"40 1","pages":"2302086"},"PeriodicalIF":2.0000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gynecological Endocrinology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/09513590.2024.2302086","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/22 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 0

Abstract

Objective: Comparison of hormonal, metabolic and inflammatory markers of glutathione with metformin and Diane-35 in a rat model of PCOS induced by dehydroepiandrosterone.

Methods: Twenty-five female rats were randomized into four groups. Group 1 was administered a subcutaneous dose of 0.2 ml saline/day. Group 2 was given 0.2 ml of 1% carboxymethyl cellulose (CMC)/day orally for 28 days. A PCOS model was established with DHEA in rats. Group 3 was given 4.5 mg/kg/day of Diane-35 orally dissolved in 1% CMC for 28 days. Group 4 was given 300 mg/kg/day of metformin orally dissolved in 1 ml of saline for 28 days, and Group 5 was administered 100 mg/kg of glutathione intraperitoneally on days 35, 42, and 49. On day 56, the rats were sacrificed. Serum markers and follicle count were examined.

Results: Serum IL-6, hs-CRP, insulin, testosterone, SHBG, and MDA values were significantly lower in the glutathione group than in the PCOS group (p = 0.0006, p = 0.023, p = 0.0082, p = 0.0007, p = 0.0048, and p < 0.0001, respectively).The number of all follicles was similar between the control and glutathione groups (p < 0.05). When we compared the other groups with the PCOS group, the number of primary, secondary, atretic, and cystic follicles was significantly lower in the metformin and glutathione groups. The number of primordial and antral follicles was significantly higher than in the PCOS group.

Conclusions: Glutathione plays anti-inflammatory and antioxidant roles, similar to metformin, by lowering serum IL-6, insulin, testosterone, CRP, and MDA levels; decreasing atretic/cystic follicle count; and improving antral follicle count and folliculogenesis in PCOS patients.

抗氧化剂谷胱甘肽与二甲双胍和 Diane-35 对 DHEA 诱导的 PCOS 大鼠模型中激素、代谢和炎症指标的比较效应。
目的在脱氢表雄酮诱导的 PCOS 大鼠模型中,比较谷胱甘肽与二甲双胍和 Diane-35 的激素、代谢和炎症指标:将 25 只雌性大鼠随机分为四组。第 1 组每天皮下注射 0.2 毫升生理盐水。第 2 组每天口服 0.2 毫升 1%羧甲基纤维素(CMC),连续 28 天。用 DHEA 建立大鼠多囊卵巢综合征模型。第 3 组大鼠每天口服 4.5 毫克/千克溶于 1% CMC 的 Diane-35,连续 28 天。第 4 组大鼠每天口服 300 毫克二甲双胍(溶于 1 毫升生理盐水),连续 28 天;第 5 组大鼠在第 35、42 和 49 天腹腔注射 100 毫克/千克谷胱甘肽。第 56 天,大鼠被处死。对血清标志物和卵泡数量进行检测:结果:谷胱甘肽组的血清 IL-6、hs-CRP、胰岛素、睾酮、SHBG 和 MDA 值明显低于 PCOS 组(P = 0.0006、P = 0.023、P = 0.0082、P = 0.0007、P = 0.0048 和 P P 结论:谷胱甘肽具有抗炎作用:谷胱甘肽具有与二甲双胍类似的抗炎和抗氧化作用,能降低多囊卵巢综合征患者的血清IL-6、胰岛素、睾酮、CRP和MDA水平;减少闭锁/囊性卵泡数量;改善前卵泡数量和卵泡生成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Gynecological Endocrinology
Gynecological Endocrinology 医学-妇产科学
CiteScore
4.40
自引率
5.00%
发文量
137
审稿时长
3-6 weeks
期刊介绍: Gynecological Endocrinology , the official journal of the International Society of Gynecological Endocrinology, covers all the experimental, clinical and therapeutic aspects of this ever more important discipline. It includes, amongst others, papers relating to the control and function of the different endocrine glands in females, the effects of reproductive events on the endocrine system, and the consequences of endocrine disorders on reproduction
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信