Mechanically stirred enzymatic membrane reactor containing HRP immobilized on Tau-SiO2@Fe3O4-GO nanocomposite for removal of tetracycline in synthetically concocted wastewater

IF 3.1 4区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biodegradation Pub Date : 2024-01-23 DOI:10.1007/s10532-023-10066-x

Monireh Besharati Vineh, Amir Ali Poostchi, Davood Kordestani, Mahmoud Karami Qushehbolagh, Ali Akbar Saboury

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Abstract

The process of mechanically stirred membrane reactor containing the suspension of horseradish peroxidase (HRP) immobilized on synthesized nanocomposite (Tau-SiO₂@Fe₃O₄-GO) was designed for continuous degradation of tetracycline. The immobilized HRP was characterized in terms of kinetic parameters and catalytic activities as these parameters were improved highly through immobilization. The stability indices including pH and temperature were investigated in parallel. The immobilized HRP was more tolerable to pH changes as compared to free HRP and the optimum temperature obtained at 40 °C. The reusability of HRP was promoted by immobilization as far as 70% of initial activity after ten cycles. The enzymatic degradation of optimum concentration of tetracycline was carried out in batch condition and 100% of tetracycline removed after 30 min. The results also showed that higher concentration of H₂O₂ exhibited more oxidation of tetracycline. The optimal ratio of HRP/H₂O₂ was also obtained at 0.005. The simultaneous process including separation and the biocatalytic degradation established in the membrane stirrer reactor concluded that no amount of tetracycline was observed in the permeate stream coming from the membrane after 30 min of operation.

Graphical abstract

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含有固定在 Tau-SiO2@Fe3O4-GO 纳米复合材料上的 HRP 的机械搅拌酶膜反应器用于去除合成废水中的四环素。

设计了一种机械搅拌膜反应器，其中含有固定在合成纳米复合材料（Tau-SiO2@Fe3O4-GO）上的辣根过氧化物酶（HRP）悬浮液，用于连续降解四环素。对固定化的 HRP 进行了动力学参数和催化活性方面的表征，因为这些参数通过固定化得到了很大的改善。同时还研究了包括 pH 值和温度在内的稳定性指标。与游离 HRP 相比，固定化 HRP 对 pH 值变化的耐受性更强，最佳温度为 40 °C。经过十次循环后，固定化 HRP 的可再利用性提高到初始活性的 70%。在批处理条件下对最佳浓度的四环素进行了酶降解，30 分钟后四环素的去除率达到 100%。结果还显示，高浓度的 H2O2 对四环素的氧化作用更大。HRP/H2O2 的最佳比例为 0.005。在膜搅拌反应器中同时进行的分离和生物催化降解过程得出结论，运行 30 分钟后，从膜流出的渗透液中没有发现任何四环素。

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来源期刊

Biodegradation 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Biodegradation publishes papers, reviews and mini-reviews on the biotransformation, mineralization, detoxification, recycling, amelioration or treatment of chemicals or waste materials by naturally-occurring microbial strains, microbial associations, or recombinant organisms. Coverage spans a range of topics, including Biochemistry of biodegradative pathways; Genetics of biodegradative organisms and development of recombinant biodegrading organisms; Molecular biology-based studies of biodegradative microbial communities; Enhancement of naturally-occurring biodegradative properties and activities. Also featured are novel applications of biodegradation and biotransformation technology, to soil, water, sewage, heavy metals and radionuclides, organohalogens, high-COD wastes, straight-, branched-chain and aromatic hydrocarbons; Coverage extends to design and scale-up of laboratory processes and bioreactor systems. Also offered are papers on economic and legal aspects of biological treatment of waste.