Styrene Production in Genetically Engineered Escherichia coli in a Two-Phase Culture.

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BioTech Pub Date : 2024-01-14 DOI:10.3390/biotech13010002

Shuhei Noda, Ryosuke Fujiwara, Yutaro Mori, Mayumi Dainin, Tomokazu Shirai, Akihiko Kondo

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Abstract

Styrene is an important industrial chemical. Although several studies have reported microbial styrene production, the amount of styrene produced in batch cultures can be increased. In this study, styrene was produced using genetically engineered Escherichia coli. First, we evaluated five types of phenylalanine ammonia lyases (PALs) from Arabidopsis thaliana (AtPAL) and Brachypodium distachyon (BdPAL) for their ability to produce trans-cinnamic acid (Cin), a styrene precursor. AtPAL2-expressing E. coli produced approximately 700 mg/L of Cin and we found that BdPALs could convert Cin into styrene. To assess styrene production, we constructed an E. coli strain that co-expressed AtPAL2 and ferulic acid decarboxylase from Saccharomyces cerevisiae. After a biphasic culture with oleyl alcohol, styrene production and yield from glucose were 3.1 g/L and 26.7% (mol/mol), respectively, which, to the best of our knowledge, are the highest values obtained in batch cultivation. Thus, this strain can be applied to the large-scale industrial production of styrene.

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基因工程大肠杆菌在两相培养中生产苯乙烯。

苯乙烯是一种重要的工业化学品。虽然已有多项研究报道了微生物苯乙烯的生产，但批量培养产生的苯乙烯量还可以增加。本研究利用基因工程大肠杆菌生产苯乙烯。首先，我们评估了拟南芥（AtPAL）和蕨类植物（BdPAL）中的五种苯丙氨酸氨裂解酶（PALs）生产苯乙烯前体反式肉桂酸（Cin）的能力。表达 AtPAL2 的大肠杆菌能产生约 700 毫克/升的 Cin，而我们发现 BdPALs 能将 Cin 转化为苯乙烯。为了评估苯乙烯的产量，我们构建了一株共同表达 AtPAL2 和来自酿酒酵母的阿魏酸脱羧酶的大肠杆菌。在与油醇进行双相培养后，苯乙烯产量和葡萄糖产量分别为 3.1 克/升和 26.7%（摩尔/摩尔），据我们所知，这是批量培养中获得的最高值。因此，该菌株可用于苯乙烯的大规模工业生产。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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